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Mendeley readers
| Chapter title |
Fluorescence-Based Methods for Characterizing RNA Interactions In Vivo
|
|---|---|
| Chapter number | 9 |
| Book title |
Bacterial Regulatory RNA
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7634-8_9 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7633-1, 978-1-4939-7634-8
|
| Authors |
Abigail N. Leistra, Mia K. Mihailovic, Lydia M. Contreras, Leistra, Abigail N., Mihailovic, Mia K., Contreras, Lydia M. |
| Abstract |
Fluorescence-based tools that measure RNA-RNA and RNA-protein interactions in vivo offer useful experimental approaches to probe the complex and dynamic physiological behavior of bacterial RNAs. Here we document the step-by-step design and application of two fluorescence-based methods for studying the regulatory interactions RNAs perform in vivo: (i) the in vivo RNA Structural Sensing System (iRS3) for measuring RNA accessibility and (ii) the trifluorescence complementation (TriFC) assay for measuring RNA-protein interactions. |
Mendeley readers
The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 12 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 4 | 33% |
| Researcher | 2 | 17% |
| Librarian | 1 | 8% |
| Student > Doctoral Student | 1 | 8% |
| Unknown | 4 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 4 | 33% |
| Chemical Engineering | 2 | 17% |
| Agricultural and Biological Sciences | 1 | 8% |
| Computer Science | 1 | 8% |
| Physics and Astronomy | 1 | 8% |
| Other | 0 | 0% |
| Unknown | 3 | 25% |