| Title |
Laboratory evolution of Pyrococcus furiosus alcohol dehydrogenase to improve the production of (2S,5S)-hexanediol at moderate temperatures
|
|---|---|
| Published in |
Extremophiles, May 2008
|
| DOI | 10.1007/s00792-008-0164-8 |
| Pubmed ID | |
| Authors |
Ronnie Machielsen, Nicole G. H. Leferink, Annemarie Hendriks, Stan J. J. Brouns, Hans-Georg Hennemann, Thomas Dauβmann, John van der Oost |
| Abstract |
There is considerable interest in the use of enantioselective alcohol dehydrogenases for the production of enantio- and diastereomerically pure diols, which are important building blocks for pharmaceuticals, agrochemicals and fine chemicals. Due to the need for a stable alcohol dehydrogenase with activity at low-temperature process conditions (30 degrees C) for the production of (2S,5S)-hexanediol, we have improved an alcohol dehydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus (AdhA). A stable S-selective alcohol dehydrogenase with increased activity at 30 degrees C on the substrate 2,5-hexanedione was generated by laboratory evolution on the thermostable alcohol dehydrogenase AdhA. One round of error-prone PCR and screening of approximately 1,500 mutants was performed. The maximum specific activity of the best performing mutant with 2,5-hexanedione at 30 degrees C was tenfold higher compared to the activity of the wild-type enzyme. A 3D-model of AdhA revealed that this mutant has one mutation in the well-conserved NADP(H)-binding site (R11L), and a second mutation (A180V) near the catalytic and highly conserved threonine at position 183. |
Mendeley readers
The data shown below were compiled from readership statistics for 60 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 60 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 16 | 27% |
| Student > Ph. D. Student | 14 | 23% |
| Student > Master | 6 | 10% |
| Professor | 5 | 8% |
| Other | 4 | 7% |
| Other | 7 | 12% |
| Unknown | 8 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 26 | 43% |
| Biochemistry, Genetics and Molecular Biology | 12 | 20% |
| Engineering | 5 | 8% |
| Chemistry | 3 | 5% |
| Earth and Planetary Sciences | 1 | 2% |
| Other | 3 | 5% |
| Unknown | 10 | 17% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 September 2018.
All research outputs
#7,453,479
of 22,786,691 outputs
Outputs from Extremophiles
#228
of 798 outputs
Outputs of similar age
#27,657
of 79,098 outputs
Outputs of similar age from Extremophiles
#2
of 6 outputs
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So far Altmetric has tracked 798 research outputs from this source. They receive a mean Attention Score of 4.2. This one is in the 42nd percentile – i.e., 42% of its peers scored the same or lower than it.
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