| Title |
ATP-binding cassette (ABC) transporters in caprine preantral follicles: gene and protein expression
|
|---|---|
| Published in |
Cell and Tissue Research, February 2018
|
| DOI | 10.1007/s00441-018-2804-3 |
| Pubmed ID | |
| Authors |
Denise Damasceno Guerreiro, Laritza Ferreira de Lima, Gildas Tetaping Mbemya, Carolina Mielgo Maside, André Marrocos Miranda, Kaio César Simiano Tavares, Benner Geraldo Alves, Luciana Rocha Faustino, Johan Smitz, José Ricardo de Figueiredo, Ana Paula Ribeiro Rodrigues |
| Abstract |
The multidrug resistance proteins ABCB1, ABCC2 and ABCG2 are an energy-dependent efflux pump that functions in systemic detoxification processes. Physiologically expressed in a variety of tissues, most abundantly in the liver and intestinal epithelia, placenta, blood-brain barrier and various stem cells, until now, these pumps were not identified in goat ovarian tissue. Therefore, the aim of this study is to analyze ABCB1, ABCC2, and ABCG2 mRNA and protein expression in goat preantral follicles. Fragments (3 × 3 × 1 mm) from five pairs of ovary (n = 10) obtained from five goat were collected and immediately submitted to qPCR, Western blot, and immunofluorescence assay for mRNA detection and identification and localization of the ABC transporters, respectively. mRNA for ABCB1, ABCC2, and ABCG2 and the presence of their proteins were observed on ovarian tissue samples. Positive marks were observed for the three transport proteins in all follicular categories studied. However, the marks were primarily localized in the oocyte of primordial, transition and primary follicle categories. In conclusion, goat ovarian tissue expresses mRNA for the ABCB1, ABCC2 and ABCG2 transporters and the expression of these proteins in the preantral follicles is a follicle-dependent stage. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 18 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 18 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 3 | 17% |
| Student > Master | 3 | 17% |
| Student > Ph. D. Student | 2 | 11% |
| Professor | 1 | 6% |
| Researcher | 1 | 6% |
| Other | 1 | 6% |
| Unknown | 7 | 39% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 4 | 22% |
| Veterinary Science and Veterinary Medicine | 2 | 11% |
| Biochemistry, Genetics and Molecular Biology | 2 | 11% |
| Medicine and Dentistry | 2 | 11% |
| Unknown | 8 | 44% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 March 2018.
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#19,236,357
of 23,839,820 outputs
Outputs from Cell and Tissue Research
#1,706
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Outputs of similar age
#259,196
of 331,833 outputs
Outputs of similar age from Cell and Tissue Research
#28
of 39 outputs
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