| Title |
Cytosolic heat-stress proteins Hsp17.7 class I and Hsp17.3 class II of tomato act as molecular chaperones in vivo
|
|---|---|
| Published in |
Planta, September 2000
|
| DOI | 10.1007/s004250000315 |
| Pubmed ID | |
| Authors |
Daniela Löw, Kurt Brändle, Lutz Nover, Christoph Forreiter |
| Abstract |
Small heat-stress proteins (sHsps) are the most abundant stress-induced proteins with up to 20 different members in higher plants. In the cytoplasm, two different classes can be distinguished. Two cDNA clones from tomato Lycopersicon peruvianum (L.) Mill., each coding for one of the cytoplasmic sHsp subfamilies, were analyzed with respect to their transcript and protein expression, genome organization and chaperone activity. Neither type was present under control conditions but both appeared upon heat stress and in mature fruits. Expression of the class II transcript was found to be induced at slightly lower temperatures than the class I transcript. Protein analysis using class-specific antibodies revealed an identical expression pattern of both corresponding proteins. Transient expression in an Arabidopsis thaliana (L.) Heynh. cell culture showed that, despite the difference in their amino acid sequence, both classes are functionally active as chaperones in vivo, as shown by their ability to prevent thermal inactivation of firefly luciferase in a cellular environment. |
Mendeley readers
The data shown below were compiled from readership statistics for 61 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 2 | 3% |
| Japan | 1 | 2% |
| Spain | 1 | 2% |
| Singapore | 1 | 2% |
| Unknown | 56 | 92% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 14 | 23% |
| Student > Ph. D. Student | 10 | 16% |
| Student > Master | 7 | 11% |
| Student > Bachelor | 5 | 8% |
| Student > Postgraduate | 4 | 7% |
| Other | 11 | 18% |
| Unknown | 10 | 16% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 37 | 61% |
| Biochemistry, Genetics and Molecular Biology | 8 | 13% |
| Engineering | 2 | 3% |
| Environmental Science | 1 | 2% |
| Chemistry | 1 | 2% |
| Other | 1 | 2% |
| Unknown | 11 | 18% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 July 2019.
All research outputs
#8,534,976
of 25,373,627 outputs
Outputs from Planta
#674
of 2,980 outputs
Outputs of similar age
#12,949
of 37,745 outputs
Outputs of similar age from Planta
#4
of 15 outputs
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