| Title |
Decrease in expression of bone morphogenetic proteins 4 and 5 in synovial tissue of patients with osteoarthritis and rheumatoid arthritis
|
|---|---|
| Published in |
Arthritis Research & Therapy, March 2006
|
| DOI | 10.1186/ar1923 |
| Pubmed ID | |
| Authors |
Carsten P Bramlage, Thomas Häupl, Christian Kaps, Ute Ungethüm, Veit Krenn, Axel Pruss, Gerhard A Müller, Frank Strutz, Gerd-R Burmester |
| Abstract |
Bone morphogenetic proteins (BMPs) have been identified as important morphogens with pleiotropic functions in regulating the development, homeostasis and repair of various tissues. The aim of this study was to characterize the expression of BMPs in synovial tissues under normal and arthritic conditions. Synovial tissue from normal donors (ND) and from patients with osteoarthritis (OA) and rheumatoid arthritis (RA) were analyzed for BMP expression by using microarray hybridization. Differential expression of BMP-4 and BMP-5 was validated by semiquantitative RT-PCR, in situ hybridization and immunohistochemistry. Activity of arthritis was determined by routine parameters for systemic inflammation, by histological scoring of synovitis and by semiquantitative RT-PCR of IL-1beta, TNF-alpha, stromelysin and collagenase I in synovial tissue. Expression of BMP-4 and BMP-5 mRNA was found to be significantly decreased in synovial tissue of patients with RA in comparison with ND by microarray analysis (p < 0.0083 and p < 0.0091). Validation by PCR confirmed these data in RA (p < 0.002) and also revealed a significant decrease in BMP-4 and BMP-5 expression in OA compared with ND (p < 0.015). Furthermore, histomorphological distribution of both morphogens as determined by in situ hybridization and immunohistochemistry showed a dominance in the lining layer of normal tissues, whereas chronically inflamed tissue from patients with RA revealed BMP expression mainly scattered across deeper layers. In OA, these changes were less pronounced with variable distribution of BMPs in the lining and sublining layer. BMP-4 and BMP-5 are expressed in normal synovial tissue and were found decreased in OA and RA. This may suggest a role of distinct BMPs in joint homeostasis that is disturbed in inflammatory and degenerative joint diseases. In comparison with previous reports, these data underline the complex impact of these factors on homeostasis and remodeling in joint physiology and pathology. |
Mendeley readers
The data shown below were compiled from readership statistics for 52 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 2 | 4% |
| Iran, Islamic Republic of | 1 | 2% |
| Netherlands | 1 | 2% |
| Unknown | 48 | 92% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 14 | 27% |
| Student > Ph. D. Student | 9 | 17% |
| Student > Master | 5 | 10% |
| Student > Doctoral Student | 5 | 10% |
| Professor > Associate Professor | 3 | 6% |
| Other | 8 | 15% |
| Unknown | 8 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 17 | 33% |
| Agricultural and Biological Sciences | 13 | 25% |
| Biochemistry, Genetics and Molecular Biology | 7 | 13% |
| Immunology and Microbiology | 2 | 4% |
| Materials Science | 2 | 4% |
| Other | 2 | 4% |
| Unknown | 9 | 17% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 March 2021.
All research outputs
#8,533,995
of 25,371,288 outputs
Outputs from Arthritis Research & Therapy
#1,710
of 3,380 outputs
Outputs of similar age
#29,362
of 85,643 outputs
Outputs of similar age from Arthritis Research & Therapy
#9
of 22 outputs
Altmetric has tracked 25,371,288 research outputs across all sources so far. This one is in the 43rd percentile – i.e., 43% of other outputs scored the same or lower than it.
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