Title |
Effects of tanshinone IIA on the hepatotoxicity and gene expression involved in alcoholic liver disease
|
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Published in |
Archives of Pharmacal Research, May 2008
|
DOI | 10.1007/s12272-001-1209-2 |
Pubmed ID | |
Authors |
Hu-Quan Yin, Youn-Su Kim, You-Jin Choi, Youn-Chul Kim, Dong-Hwan Sohn, Shi-Yong Ryu, Byung-Hoon Lee |
Abstract |
Tanshinone IIA is one of the most abundant constituents of the root of Salvia miltiorrhiza BUNGE which exerts antioxidant and anti-inflammatory actions in many experimental disease models. In the present study, we demonstrated that the standardized fraction of S. miltiorrhiza (Sm-SF) was able to protect RAW 264.7 cells from ethanol-and lipopolysaccharide (LPS)-induced production of superoxide radical, activation of NADPH oxidase and subsequently death of the cells. Among four main components of Sm-SF, tanshinone IIA was the most potent in protecting cells from LPS-and ethanol-induced cytotoxicity. LPS or ethanol induced the expression of CD14, iNOS, and SCD1 and decreased RXR-alpha, which was completely reversed by tanshinone IIA. In H4IIEC3 cells, 10 microM tanshinone IIA effectively blocked ethanol-induced fat accumulation as evidenced by Nile Red binding assay. These results indicate that tanshinone IIA may have potential to inhibit alcoholic liver disease by reducing LPS-and ethanol-induced Kupffer cell sensitization, inhibiting synthesis of reactive oxygen/nitrogen species, inhibiting fatty acid synthesis and stimulating fatty acid oxidation. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 10 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Professor | 2 | 20% |
Student > Postgraduate | 2 | 20% |
Student > Ph. D. Student | 2 | 20% |
Other | 1 | 10% |
Student > Doctoral Student | 1 | 10% |
Other | 2 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Pharmacology, Toxicology and Pharmaceutical Science | 3 | 30% |
Agricultural and Biological Sciences | 3 | 30% |
Medicine and Dentistry | 2 | 20% |
Unknown | 2 | 20% |