| Title |
Structure and expression of the gene encoding the periplasmic arylsulfatase of Chlamydomonas reinhardtii
|
|---|---|
| Published in |
Molecular Genetics and Genomics, August 1989
|
| DOI | 10.1007/bf00331273 |
| Pubmed ID | |
| Authors |
Eugenio L. de Hostos, James Schilling, Arthur R. Grossman |
| Abstract |
Chlamydomonas reinhardtii produces a periplasmic arylsulfatase in response to sulfur deprivation. We have isolated and sequenced arylsulfatase cDNAs from a lambda gt11 expression library. The amino acid sequence of the protein, as deduced from the nucleotide sequence, has features characteristic of secreted proteins, including a signal sequence and putative glycosylation sites. The gene has a broad codon usage with seven codons, all having A residues in the third position, not previously observed in C. reinhardtii genes. Arylsulfatase transcription is tightly regulated by sulfur availability. The approximately 2.7 kb arylsulfatase transcript is very susceptible to degradation, disappearing in less than an hour after sulfur starved cells are administered either sulfate or alpha-amanitin. The accumulation of the arylsulfatase transcript is also suppressed by the addition of cycloheximide. Transcription initiation from the arylsulfatase gene occurs approximately 100 bp upstream of the initiation codon, in a region that is 5' to a 43 bp imperfect inverted repeat. Preceding the transcription start site are sequences similar to those present in promoter regions of other genes from C. reinhardtii. |
Mendeley readers
The data shown below were compiled from readership statistics for 30 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | 3% |
| Canada | 1 | 3% |
| Unknown | 28 | 93% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Professor | 7 | 23% |
| Researcher | 7 | 23% |
| Student > Bachelor | 5 | 17% |
| Student > Ph. D. Student | 4 | 13% |
| Student > Master | 2 | 7% |
| Other | 2 | 7% |
| Unknown | 3 | 10% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 12 | 40% |
| Biochemistry, Genetics and Molecular Biology | 10 | 33% |
| Environmental Science | 2 | 7% |
| Engineering | 2 | 7% |
| Nursing and Health Professions | 1 | 3% |
| Other | 1 | 3% |
| Unknown | 2 | 7% |
Attention Score in Context
This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 August 2021.
All research outputs
#5,446,994
of 25,374,647 outputs
Outputs from Molecular Genetics and Genomics
#321
of 3,318 outputs
Outputs of similar age
#1,685
of 13,593 outputs
Outputs of similar age from Molecular Genetics and Genomics
#6
of 27 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. Compared to these this one has done well and is in the 75th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 3,318 research outputs from this source. They receive a mean Attention Score of 4.0. This one has done well, scoring higher than 76% of its peers.
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We're also able to compare this research output to 27 others from the same source and published within six weeks on either side of this one. This one is in the 48th percentile – i.e., 48% of its contemporaries scored the same or lower than it.