| Title |
Speciation analysis of arsenic in seafood and seaweed: Part II—single laboratory validation of method
|
|---|---|
| Published in |
Analytical & Bioanalytical Chemistry, February 2018
|
| DOI | 10.1007/s00216-018-0910-4 |
| Pubmed ID | |
| Authors |
Mesay Mulugeta Wolle, Sean D. Conklin |
| Abstract |
Single laboratory validation of a method for arsenic speciation analysis in seafood and seaweed is presented. The method is based on stepwise extraction of water-soluble and non-polar arsenic with hot water and a mixture of dichloromethane and methanol, respectively. While the water-soluble arsenicals were speciated by anion and cation exchange liquid chromatography inductively coupled plasma mass spectrometry (LC-ICP-MS), the non-polar arsenicals were collectively determined by ICP-MS after digestion in acid. The performance characteristics and broad application of the method were evaluated by analyzing eight commercial samples (cod, haddock, mackerel, crab, shrimp, geoduck clam, oyster, and kombu) and four reference materials (fish protein (DORM-4), lobster hepatopancreas (TORT-3), mussel tissue (SRM 2976), and hijiki seaweed (CRM 7405-a)) representing finfish, crustaceans, molluscs, and seaweed. Matrices spiked at three levels in duplicates were also analyzed. The stepwise extraction provided 76-106% extraction of the total arsenic from the test materials. The method demonstrated satisfactory repeatability for analysis of replicate extracts prepared over several days. The accuracy of the method was evaluated by analyzing reference materials certified for both total arsenic and a few arsenicals; the experimental results were 90-105% of the certified values. Comparison between the total water-soluble arsenic and the sum of the concentrations of the chromatographed species gave 80-92% mass balance. While spike recoveries of most arsenicals were in the acceptance range set by CODEX, a few species spiked into cod, haddock, and shrimp were poorly recovered due to transformation to other forms. After thorough investigations, strategies were devised to improve the recoveries of these species by averting their transformations. Limits of quantification (LOQ) for the extraction and quantification of 16 arsenicals using the current method were in the range 6-16 ng g-1arsenic. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 64 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 64 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 7 | 11% |
| Student > Master | 7 | 11% |
| Student > Bachelor | 7 | 11% |
| Researcher | 6 | 9% |
| Lecturer | 4 | 6% |
| Other | 9 | 14% |
| Unknown | 24 | 38% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 12 | 19% |
| Agricultural and Biological Sciences | 11 | 17% |
| Environmental Science | 3 | 5% |
| Nursing and Health Professions | 2 | 3% |
| Medicine and Dentistry | 2 | 3% |
| Other | 5 | 8% |
| Unknown | 29 | 45% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 August 2018.
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#20,663,600
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Outputs from Analytical & Bioanalytical Chemistry
#6,602
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#268,709
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Outputs of similar age from Analytical & Bioanalytical Chemistry
#121
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