| Title |
The use of percoll gradients, elutriator rotor elution, and mithramycin staining for the isolation and identification of intraerythrocytic stages ofPlasmodium berghei
|
|---|---|
| Published in |
Zeitschrift für Parasitenkunde, October 1982
|
| DOI | 10.1007/bf00925344 |
| Pubmed ID | |
| Authors |
Lorenz Rüssmann, Albrecht Jung, Hans-G. Heidrich |
| Abstract |
Intraerythrocytic parasites of Plasmodium vinckei and Plasmodium berghei were separated according to their developmental stages using discontinuous Percoll gradients. Contaminating nucleated blood cells such as leukocytes were removed by elutriation centrifugation. The stages were unequivocally identified in smears using a newly developed DNA-specific staining procedure with mithramycin and fluorescence microscopy. This stain can also be used to detect parasites in human blood of very low parasitemias. The combination of methods described has many possible applications in immunologic and biochemical parasite research. |
Mendeley readers
The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Canada | 1 | 13% |
| Unknown | 7 | 88% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 3 | 38% |
| Student > Master | 2 | 25% |
| Student > Doctoral Student | 1 | 13% |
| Professor | 1 | 13% |
| Other | 1 | 13% |
| Other | 0 | 0% |
| Readers by discipline | Count | As % |
|---|---|---|
| Veterinary Science and Veterinary Medicine | 1 | 13% |
| Environmental Science | 1 | 13% |
| Biochemistry, Genetics and Molecular Biology | 1 | 13% |
| Agricultural and Biological Sciences | 1 | 13% |
| Medicine and Dentistry | 1 | 13% |
| Other | 2 | 25% |
| Unknown | 1 | 13% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 August 2014.
All research outputs
#8,535,472
of 25,374,647 outputs
Outputs from Zeitschrift für Parasitenkunde
#16
of 94 outputs
Outputs of similar age
#2,171
of 7,631 outputs
Outputs of similar age from Zeitschrift für Parasitenkunde
#1
of 4 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. This one is in the 43rd percentile – i.e., 43% of other outputs scored the same or lower than it.
So far Altmetric has tracked 94 research outputs from this source. They receive a mean Attention Score of 4.1. This one is in the 8th percentile – i.e., 8% of its peers scored the same or lower than it.
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