| Title |
Isolation and characterization of indene bioconversion genes from Rhodococcus strain I24
|
|---|---|
| Published in |
Applied Microbiology and Biotechnology, June 1999
|
| DOI | 10.1007/s002530051463 |
| Pubmed ID | |
| Authors |
S. L. Treadway, K. S. Yanagimachi, E. Lankenau, P. A. Lessard, G. Stephanopoulos, A. J. Sinskey |
| Abstract |
Rhodococcus strain 124 is able to convert indene into indandiol via the actions of at least two dioxygenase systems and a putative monooxygenase system. We have identified a cosmid clone from 124 genomic DNA that is able to confer the ability to convert indene to indandiol upon Rhodococcus erythropolis SQ1, a strain that normally can not convert or metabolize indene. HPLC analysis reveals that the transformed SQ1 strain produces cis-(1R,2S)-indandiol, suggesting that the cosmid clone encodes a naphthalenetype dioxygenase. DNA sequence analysis of a portion of this clone confirmed the presence of genes for the dioxygenase as well as genes encoding a dehydrogenase and putative aldolase. These genes will be useful for manipulating indene bioconversion in Rhodococcus strain 124. |
Mendeley readers
The data shown below were compiled from readership statistics for 40 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 40 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 9 | 23% |
| Student > Bachelor | 7 | 18% |
| Student > Master | 5 | 13% |
| Researcher | 4 | 10% |
| Professor | 3 | 8% |
| Other | 4 | 10% |
| Unknown | 8 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 16 | 40% |
| Biochemistry, Genetics and Molecular Biology | 5 | 13% |
| Environmental Science | 2 | 5% |
| Chemistry | 2 | 5% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 3% |
| Other | 4 | 10% |
| Unknown | 10 | 25% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 December 2014.
All research outputs
#8,534,528
of 25,371,288 outputs
Outputs from Applied Microbiology and Biotechnology
#2,898
of 8,290 outputs
Outputs of similar age
#11,564
of 35,791 outputs
Outputs of similar age from Applied Microbiology and Biotechnology
#12
of 24 outputs
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