| Title |
Different concentrations of berberine result in distinct cellular localization patterns and cell cycle effects in a melanoma cell line
|
|---|---|
| Published in |
Cancer Chemotherapy and Pharmacology, July 2007
|
| DOI | 10.1007/s00280-007-0558-9 |
| Pubmed ID | |
| Authors |
Teresa L. Serafim, Paulo J. Oliveira, Vilma A. Sardao, Ed Perkins, Donna Parke, Jon Holy |
| Abstract |
Natural products represent a rich reservoir of potential small molecule inhibitors exhibiting antiproliferative and tumoricidal properties. An example is the isoquinoline alkaloid berberine, which is found in plants such as goldenseal (Hydrastis canadensis). Studies have shown that berberine is able to trigger apoptosis in different malignant cell lines, and can also lead to cell cycle arrest at sub-apoptotic doses. A particularly interesting feature of berberine is the fact that it is a fluorescent molecule, and its uptake and distribution in cells can be studied by flow cytometry and epifluorescence microscopy. To test the relationships between berberine uptake, distribution and cellular effect in melanoma cells, K1735-M2 mouse and WM793 human melanoma cells were treated with different concentrations of berberine, and alterations in cell cycle progression, DNA synthesis, cell proliferation, and cell death measured. |
Mendeley readers
The data shown below were compiled from readership statistics for 54 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Portugal | 2 | 4% |
| India | 1 | 2% |
| Japan | 1 | 2% |
| Unknown | 50 | 93% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 11 | 20% |
| Researcher | 9 | 17% |
| Professor | 5 | 9% |
| Professor > Associate Professor | 4 | 7% |
| Student > Master | 4 | 7% |
| Other | 10 | 19% |
| Unknown | 11 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 12 | 22% |
| Biochemistry, Genetics and Molecular Biology | 8 | 15% |
| Immunology and Microbiology | 6 | 11% |
| Chemistry | 4 | 7% |
| Medicine and Dentistry | 4 | 7% |
| Other | 4 | 7% |
| Unknown | 16 | 30% |
Attention Score in Context
This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 December 2017.
All research outputs
#3,113,074
of 23,815,455 outputs
Outputs from Cancer Chemotherapy and Pharmacology
#100
of 2,501 outputs
Outputs of similar age
#7,328
of 68,460 outputs
Outputs of similar age from Cancer Chemotherapy and Pharmacology
#1
of 18 outputs
Altmetric has tracked 23,815,455 research outputs across all sources so far. Compared to these this one has done well and is in the 86th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,501 research outputs from this source. They receive a mean Attention Score of 4.1. This one has done particularly well, scoring higher than 95% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 68,460 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 88% of its contemporaries.
We're also able to compare this research output to 18 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 94% of its contemporaries.