| Title |
Isolation and characterization of sulfur globule proteins from Chromatium vinosum and Thiocapsa roseopersicina
|
|---|---|
| Published in |
Archives of Microbiology, June 1995
|
| DOI | 10.1007/bf00272127 |
| Pubmed ID | |
| Authors |
Daniel C. Brune |
| Abstract |
Purple sulfur bacteria store sulfur as intracellular globules enclosed by a protein envelope. The proteins associated with sulfur globules of Chromatium vinosum and Thiocapsa roseopersicina were isolated by extraction into 50% aqueous acetonitrile containing 1% trifluoroacetic acid and 10 mM dithiothreitol. The extracted proteins were separated by reversed-phase HPLC, revealing three major proteins from C. vinosum and two from T. roseopersicina. All of these proteins have similar, rather unusual amino acid compositions, being rich in glycine and aromatic amino acids, particularly tyrosine. The molecular masses of the C. vinosum proteins were determined to be 10,498, 10,651, and 8,479 Da, while those from T. roseopersicina were found to be 10,661 and 8,759 Da by laser desorption time-of-flight mass spectrometry. The larger T. roseopersicina protein is N-terminally blocked, probably by acetylation, but small amounts of the unblocked form (mass = 10,619) were also isolated by HPLC. Protein sequencing showed that the two larger C. vinosum proteins are homologous to each other and to the large T. roseopersicina protein. The 8,479 Da C. vinosum and 8,759 Da T. roseopersicina proteins are also homologous, indicating that sulfur globule proteins are conserved between different species of purple sulfur bacteria. |
Mendeley readers
The data shown below were compiled from readership statistics for 33 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | 3% |
| Unknown | 32 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 8 | 24% |
| Student > Bachelor | 3 | 9% |
| Student > Doctoral Student | 3 | 9% |
| Student > Postgraduate | 3 | 9% |
| Researcher | 3 | 9% |
| Other | 5 | 15% |
| Unknown | 8 | 24% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 9 | 27% |
| Biochemistry, Genetics and Molecular Biology | 3 | 9% |
| Chemistry | 2 | 6% |
| Immunology and Microbiology | 2 | 6% |
| Engineering | 2 | 6% |
| Other | 6 | 18% |
| Unknown | 9 | 27% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 January 2022.
All research outputs
#7,483,725
of 22,875,477 outputs
Outputs from Archives of Microbiology
#569
of 2,783 outputs
Outputs of similar age
#7,436
of 24,939 outputs
Outputs of similar age from Archives of Microbiology
#6
of 11 outputs
Altmetric has tracked 22,875,477 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,783 research outputs from this source. They receive a mean Attention Score of 3.7. This one is in the 43rd percentile – i.e., 43% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 24,939 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 8th percentile – i.e., 8% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 11 others from the same source and published within six weeks on either side of this one. This one is in the 9th percentile – i.e., 9% of its contemporaries scored the same or lower than it.