| Title |
Detection of HPV infection in head and neck squamous cell carcinoma: a practical proposal
|
|---|---|
| Published in |
Virchows Archiv, March 2013
|
| DOI | 10.1007/s00428-013-1393-5 |
| Pubmed ID | |
| Authors |
Johannes H. Dreyer, Franziska Hauck, Michelle Oliveira-Silva, Mario Henrique M. Barros, Gerald Niedobitek |
| Abstract |
Detecting human papillomavirus (HPV) infection in head and neck squamous cell carcinoma (HNSCC) is clinically relevant, but there is no agreement about the most appropriate methodology. We have studied 64 oropharyngeal carcinomas using p16 immunohistochemistry, HPV DNA in situ hybridisation (ISH) and HPV DNA polymerase chain reaction (PCR) followed by pyrosequencing. We have also evaluated a new assay, RNAscope, designed to detect HPV E6/E7 RNA transcripts. Using a threshold of 70 % labelled tumour cells, 21 cases (32.8 %) were p16 positive. Of these, 19 cases scored positive with at least one HPV detection assay. Sixteen cases were positive by HPV DNA-ISH, and 18 cases were positive using the E6/E7 RNAscope assay. By PCR and pyrosequencing, HPV16 was detected in 15 cases, while one case each harboured HPV33, 35 and 56. All p16-negative cases were negative using these assays. We conclude that p16 expression is a useful surrogate marker for HPV infection in HNSCC with a high negative predictive value and that p16-positive cases should be further evaluated for HPV infection, preferably by PCR followed by type determination. Using RNase digestion experiments, we show that the RNAscope assay is not suitable for the reliable discrimination between E6/E7 RNA transcripts and viral DNA. |
Mendeley readers
The data shown below were compiled from readership statistics for 47 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Brazil | 1 | 2% |
| Unknown | 46 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 10 | 21% |
| Student > Doctoral Student | 6 | 13% |
| Other | 6 | 13% |
| Researcher | 6 | 13% |
| Student > Bachelor | 4 | 9% |
| Other | 8 | 17% |
| Unknown | 7 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 26 | 55% |
| Agricultural and Biological Sciences | 9 | 19% |
| Biochemistry, Genetics and Molecular Biology | 2 | 4% |
| Computer Science | 1 | 2% |
| Nursing and Health Professions | 1 | 2% |
| Other | 2 | 4% |
| Unknown | 6 | 13% |
Attention Score in Context
This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 February 2016.
All research outputs
#4,696,232
of 22,787,797 outputs
Outputs from Virchows Archiv
#177
of 1,950 outputs
Outputs of similar age
#44,099
of 216,352 outputs
Outputs of similar age from Virchows Archiv
#3
of 14 outputs
Altmetric has tracked 22,787,797 research outputs across all sources so far. Compared to these this one has done well and is in the 76th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,950 research outputs from this source. They receive a mean Attention Score of 4.0. This one has done well, scoring higher than 88% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 216,352 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 77% of its contemporaries.
We're also able to compare this research output to 14 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 78% of its contemporaries.