| Title |
Tissue distribution of the secretory protein, SPLUNC1, in the human fetus
|
|---|---|
| Published in |
Histochemistry and Cell Biology, September 2005
|
| DOI | 10.1007/s00418-005-0070-4 |
| Pubmed ID | |
| Authors |
Hou-De Zhou, Song-Qin Fan, Jin Zhao, Dong-Hai Huang, Ming Zhou, Hua-Ying Liu, Zhao-Yang Zeng, Yi-Xing Yang, He Huang, Xiao-Ling Li, Shou-Rong Shen, Gui-Yuan Li |
| Abstract |
We previously identified a tissue-specific gene, short palate, lung, and nasal epithelium clone 1 (SPLUNC1), in nasopharyngeal epithelial tissues. SPLUNC1 was differentially expressed in nasopharyngeal carcinoma. Bioinformatic analysis revealed that SPLUNC1 has the bactericidal permeability-increasing protein/lipid-binding protein (BPI/LBP) domain and a 19 amino acid signal peptide, which suggest that it is a secretory protein. Its precise cellular localization in the respiratory tract is mainly in mucous cells and ducts of submucosal glands. However, little is known about its expression pattern in various human tissues. We generated a highly specific antibody and analyzed its distribution in the human fetus by immunohistochemistry to more precisely determine SPLUNC1 protein localization in human tissues. The results were further validated by RT-PCR. Our results showed that SPLUNC1 protein is expressed at not only the serous glands and epithelium of the upper respiratory tract and digestive tract, but also in the oculi of human embryos. Interestingly, we also found positive staining in fetus adipose tissue, a result not previously reported in studies of adult human tissues. Western blot analysis detected a 24 kDa SPLUNC1 protein in the compounds of nasopharyngeal secretions. This secretory protein was also detected in saliva and tears. Our research suggests that SPLUNC1 protein may not only be an antimicrobial peptide that plays an important role in the maintenance of homeostasis in the upper respiratory tract, oculi, and alimentary tract, it may also be important in the development and lipid metabolism of the adipose tissue. |
Mendeley readers
The data shown below were compiled from readership statistics for 14 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 1 | 7% |
| Unknown | 13 | 93% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 4 | 29% |
| Student > Doctoral Student | 2 | 14% |
| Student > Bachelor | 2 | 14% |
| Student > Master | 2 | 14% |
| Student > Ph. D. Student | 1 | 7% |
| Other | 2 | 14% |
| Unknown | 1 | 7% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 5 | 36% |
| Agricultural and Biological Sciences | 4 | 29% |
| Biochemistry, Genetics and Molecular Biology | 2 | 14% |
| Immunology and Microbiology | 1 | 7% |
| Chemistry | 1 | 7% |
| Other | 0 | 0% |
| Unknown | 1 | 7% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 March 2010.
All research outputs
#8,064,660
of 24,217,893 outputs
Outputs from Histochemistry and Cell Biology
#247
of 926 outputs
Outputs of similar age
#21,319
of 60,353 outputs
Outputs of similar age from Histochemistry and Cell Biology
#4
of 12 outputs
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