| Title |
Developmental validation of GlobalFiler™ PCR amplification kit: a 6-dye multiplex assay designed for amplification of casework samples
|
|---|---|
| Published in |
International Journal of Legal Medicine, March 2018
|
| DOI | 10.1007/s00414-018-1817-5 |
| Pubmed ID | |
| Authors |
Matthew J. Ludeman, Chang Zhong, Julio J. Mulero, Robert E. Lagacé, Lori K. Hennessy, Marc L. Short, Dennis Y. Wang |
| Abstract |
The GlobalFiler™ PCR Amplification Kit is a single multiplex assay that amplifies a set of 24 markers, which encompass the European Standard Set and CODIS (Combined DNA Index System) recommended composite set of loci. In addition to more loci and a 6-dye chemistry format, the Master Mix has been formulated to allow higher sample loading volume for trace DNA samples. The GlobalFiler™ Kit has been optimized to deliver high performance on casework samples, while also delivering fast thermal cycling, with an amplification time of approximately 80 min. Here, we report the results of the developmental validation study which followed the SWGDAM (Scientific Working Group on DNA Analysis Methods) guidelines and includes data for PCR-based studies, sensitivity, species specificity, stability, precision, reproducibility and repeatability, concordance, stutter, DNA mixtures, and performance on mock casework samples. The results validate the multiplex design as well as demonstrate the kit's robustness, reliability, and suitability as an assay for human identification with casework DNA samples. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Spain | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 124 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 124 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 20 | 16% |
| Student > Master | 18 | 15% |
| Student > Bachelor | 13 | 10% |
| Researcher | 10 | 8% |
| Other | 6 | 5% |
| Other | 13 | 10% |
| Unknown | 44 | 35% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 47 | 38% |
| Agricultural and Biological Sciences | 11 | 9% |
| Medicine and Dentistry | 7 | 6% |
| Chemistry | 4 | 3% |
| Social Sciences | 2 | 2% |
| Other | 4 | 3% |
| Unknown | 49 | 40% |
Attention Score in Context
This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 June 2023.
All research outputs
#4,883,459
of 24,081,774 outputs
Outputs from International Journal of Legal Medicine
#231
of 2,164 outputs
Outputs of similar age
#91,687
of 335,829 outputs
Outputs of similar age from International Journal of Legal Medicine
#6
of 61 outputs
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