| Title |
Ex vivo immunomodulatory effect of ethanolic extract of propolis during Celiac Disease: involvement of nitric oxide pathway
|
|---|---|
| Published in |
Inflammopharmacology, March 2018
|
| DOI | 10.1007/s10787-018-0460-6 |
| Pubmed ID | |
| Authors |
Oussama Medjeber, Kahina Touri, Hayet Rafa, Zineb Djeraba, Mourad Belkhelfa, Amira Fatima Boutaleb, Amina Arroul-Lammali, Houda Belguendouz, Chafia Touil-Boukoffa |
| Abstract |
Celiac Disease (CeD) is a chronic immune-mediated enteropathy, in which dietary gluten induces an inflammatory reaction, predominantly in the duodenum. Propolis is a resinous hive product, collected by honeybees from various plant sources. Propolis is well-known for its anti-inflammatory, anti-oxidant and immunomodulatory effects, due to its major compounds, polyphenols and flavonoids. The aim of our study was to assess the ex vivo effect of ethanolic extract of propolis (EEP) upon the activity and expression of iNOS, along with IFN-γ and IL-10 production in Algerian Celiac patients. In this context, PBMCs isolated from peripheral blood of Celiac patients and healthy controls were cultured with different concentrations of EEP. NO production was measured using the Griess method, whereas quantitation of IFN-γ and IL-10 levels was performed by ELISA. Inducible nitric oxide synthase (iNOS) expression, NFκB and pSTAT-3 activity were analyzed by immunofluorescence assay. Our results showed that PBMCs from Celiac patients produced high levels of NO and IFN-γ compared with healthy controls (HC). Interestingly, EEP reduced significantly, NO and IFN-γ levels and significantly increased IL-10 levels at a concentration of 50 µg/mL. Importantly, EEP downmodulated the iNOS expression as well as the activity of NFκB and pSTAT-3 transcription factors. Altogether, our results highlight the immunomodulatory effect of propolis on NO pathway and on pro-inflammatory cytokines. Therefore, we suggest that propolis may constitute a potential candidate to modulate inflammation during Celiac Disease and has a potential therapeutic value. |
X Demographics
The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 3 | 75% |
| Unknown | 1 | 25% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 75% |
| Practitioners (doctors, other healthcare professionals) | 1 | 25% |
Mendeley readers
The data shown below were compiled from readership statistics for 43 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 43 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Unspecified | 7 | 16% |
| Researcher | 6 | 14% |
| Student > Bachelor | 5 | 12% |
| Student > Master | 4 | 9% |
| Student > Doctoral Student | 3 | 7% |
| Other | 3 | 7% |
| Unknown | 15 | 35% |
| Readers by discipline | Count | As % |
|---|---|---|
| Unspecified | 7 | 16% |
| Biochemistry, Genetics and Molecular Biology | 5 | 12% |
| Immunology and Microbiology | 4 | 9% |
| Chemistry | 3 | 7% |
| Medicine and Dentistry | 3 | 7% |
| Other | 6 | 14% |
| Unknown | 15 | 35% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 December 2023.
All research outputs
#15,008,320
of 25,516,314 outputs
Outputs from Inflammopharmacology
#351
of 679 outputs
Outputs of similar age
#180,568
of 348,401 outputs
Outputs of similar age from Inflammopharmacology
#9
of 22 outputs
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