| Title |
Aberrant GSH reductase and NOX activities concur with defective CFTR to pro-oxidative imbalance in cystic fibrosis airways
|
|---|---|
| Published in |
Journal of Bioenergetics and Biomembranes, March 2018
|
| DOI | 10.1007/s10863-018-9748-x |
| Pubmed ID | |
| Authors |
L. de Bari, M. Favia, A. Bobba, R. Lassandro, L. Guerra, A. Atlante |
| Abstract |
Cystic fibrosis (CF) is associated to impaired Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) channel also causing decreased glutathione (GSH) secretion, defective airway bacterial clearance and inflammation. Here we checked the main ROS-producing and ROS-scavenging enzymes as potential additional factors involved in CF pathogenesis. We found that CFBE41o-cells, expressing F508del CFTR, have increased NADPH oxidase (NOX) activity and expression level, mainly responsible of the increased ROS production, and decreased glutathione reductase (GR) activity, not dependent on GR protein level decrease. Furthermore, defective CFTR proved to cause both extracellular and intracellular GSH level decrease, probably by reducing the amount of extracellular GSH-derived cysteine required for cytosolic GSH synthesis. Importantly, we provide evidence that defective CFTR and NOX/GR activity imbalance both contribute to NADPH and GSH level decrease and ROS overproduction in CF cells. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 22 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 22 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 4 | 18% |
| Student > Doctoral Student | 3 | 14% |
| Student > Bachelor | 3 | 14% |
| Student > Ph. D. Student | 3 | 14% |
| Student > Master | 3 | 14% |
| Other | 2 | 9% |
| Unknown | 4 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 5 | 23% |
| Immunology and Microbiology | 4 | 18% |
| Medicine and Dentistry | 3 | 14% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 5% |
| Computer Science | 1 | 5% |
| Other | 3 | 14% |
| Unknown | 5 | 23% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 March 2018.
All research outputs
#16,188,009
of 23,867,274 outputs
Outputs from Journal of Bioenergetics and Biomembranes
#320
of 466 outputs
Outputs of similar age
#216,299
of 335,413 outputs
Outputs of similar age from Journal of Bioenergetics and Biomembranes
#5
of 7 outputs
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So far Altmetric has tracked 466 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 25th percentile – i.e., 25% of its peers scored the same or lower than it.
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We're also able to compare this research output to 7 others from the same source and published within six weeks on either side of this one. This one has scored higher than 2 of them.