| Title |
Air synthetase in cowpea nodules: a single gene product targeted to two organelles?
|
|---|---|
| Published in |
Plant Molecular Biology, April 1998
|
| DOI | 10.1023/a:1005969830314 |
| Pubmed ID | |
| Authors |
Penelope M.C. Smith, Anthea J. Mann, Danica E. Goggin, Craig A. Atkins |
| Abstract |
A cDNA (VUpur5) encoding phosphoribosyl aminoimidazole (AIR) synthetase, the fifth enzyme of the de novo purine biosynthesis pathway has been isolated from a cowpea nodule cDNA library. It encodes a 388 amino acid protein with a predicted molecular mass of 40.4 kDa. The deduced amino acid sequence has significant homology with AIR synthetase from other organisms. AIR synthetase is present in both mitochondria and plastids of cowpea nodules. A signal sequence encoded by the VUpur5 cDNA has properties associated with plastid transit sequences but there is no consensus cleavage site as would be expected for a plastid targeted protein. Although the signal sequence does not have the structural features of a mitochondrial targeted protein, it has a mitochondrial cleavage site motif (RX/XS) close to the predicted N-terminus of the mature protein. Southern analysis suggests that AIR synthetase is encoded by a single gene raising questions as to how the product of this gene is targeted to the two organelles. VUpur5 is expressed at much higher levels in nodules compared to other cowpea tissues and the gene is active before nitrogen fixation begins. These results suggest that products of nitrogen fixation do not play a role in the initial induction of gene expression. VUpur5 was expressed in Escherichia coli and the recombinant protein used to raise antibodies. These antibodies recognize two forms of AIR synthetase which differ in molecular size. Both forms are present in mitochondria, although the larger protein is more abundant. Only the smaller protein was detected in plastids. |
Mendeley readers
The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 10% |
| China | 1 | 10% |
| Unknown | 8 | 80% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 5 | 50% |
| Professor > Associate Professor | 2 | 20% |
| Professor | 1 | 10% |
| Student > Doctoral Student | 1 | 10% |
| Unknown | 1 | 10% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 7 | 70% |
| Biochemistry, Genetics and Molecular Biology | 2 | 20% |
| Unknown | 1 | 10% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 February 2022.
All research outputs
#8,534,528
of 25,371,288 outputs
Outputs from Plant Molecular Biology
#1,017
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Outputs of similar age
#10,369
of 32,427 outputs
Outputs of similar age from Plant Molecular Biology
#5
of 11 outputs
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