| Title |
Specificity of blebbistatin, an inhibitor of myosin II
|
|---|---|
| Published in |
Journal of Muscle Research and Cell Motility, August 2004
|
| DOI | 10.1007/s10974-004-6060-7 |
| Pubmed ID | |
| Authors |
John Limouze, Aaron F. Straight, Timothy Mitchison, James R. Sellers |
| Abstract |
Blebbistatin is a small molecule inhibitor discovered in a screen for inhibitors of nonmuscle myosin IIA. We have examined the specificity and potency of the drug by assaying its effects on the actin-activated MgATPase assay of diverse members of the myosin superfamily. Blebbistatin potently inhibits several striated muscle myosins as well as vertebrate nonmuscle myosin IIA and IIB with IC50 values ranging from 0.5 to 5 microM. Interestingly, smooth muscle which is highly homologous to vertebrate nonmuscle myosin is only poorly inhibited (IC50=80 microM). The drug potently inhibits Dictyostelium myosin II, but poorly inhibits Acanthamoeba myosin II. Blebbistatin did not inhibit representative myosin superfamily members from classes I, V, and X. |
Mendeley readers
The data shown below were compiled from readership statistics for 264 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 5 | 2% |
| Germany | 2 | <1% |
| France | 1 | <1% |
| United Kingdom | 1 | <1% |
| India | 1 | <1% |
| Japan | 1 | <1% |
| Spain | 1 | <1% |
| Unknown | 252 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 77 | 29% |
| Researcher | 49 | 19% |
| Student > Master | 23 | 9% |
| Student > Bachelor | 22 | 8% |
| Professor > Associate Professor | 19 | 7% |
| Other | 43 | 16% |
| Unknown | 31 | 12% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 100 | 38% |
| Biochemistry, Genetics and Molecular Biology | 40 | 15% |
| Medicine and Dentistry | 23 | 9% |
| Engineering | 19 | 7% |
| Physics and Astronomy | 11 | 4% |
| Other | 29 | 11% |
| Unknown | 42 | 16% |
Attention Score in Context
This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 October 2022.
All research outputs
#3,798,945
of 25,374,917 outputs
Outputs from Journal of Muscle Research and Cell Motility
#12
of 301 outputs
Outputs of similar age
#6,306
of 61,346 outputs
Outputs of similar age from Journal of Muscle Research and Cell Motility
#1
of 6 outputs
Altmetric has tracked 25,374,917 research outputs across all sources so far. Compared to these this one has done well and is in the 83rd percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 301 research outputs from this source. They receive a mean Attention Score of 3.1. This one has done particularly well, scoring higher than 95% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 61,346 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 85% of its contemporaries.
We're also able to compare this research output to 6 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them