Chapter title |
Chromatin Immunoprecipitation Sequencing (ChIP-Seq) for Transcription Factors and Chromatin Factors in Arabidopsis thaliana Roots: From Material Collection to Data Analysis
|
---|---|
Chapter number | 18 |
Book title |
Root Development
|
Published in |
Methods in molecular biology, March 2018
|
DOI | 10.1007/978-1-4939-7747-5_18 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7746-8, 978-1-4939-7747-5
|
Authors |
Sandra Cortijo, Varodom Charoensawan, François Roudier, Philip A. Wigge, Cortijo, Sandra, Charoensawan, Varodom, Roudier, François, Wigge, Philip A. |
Abstract |
Chromatin immunoprecipitation combined with next-generation sequencing (ChIP-seq) is a powerful technique to investigate in vivo transcription factor (TF) binding to DNA, as well as chromatin marks. Here we provide a detailed protocol for all the key steps to perform ChIP-seq in Arabidopsis thaliana roots, also working on other A. thaliana tissues and in most non-ligneous plants. We detail all steps from material collection, fixation, chromatin preparation, immunoprecipitation, library preparation, and finally computational analysis based on a combination of publicly available tools. |
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