| Title |
COX-1 is coupled with mPGES-1 and ABCC4 in human cervix cancer cells
|
|---|---|
| Published in |
Molecular and Cellular Biochemistry, April 2009
|
| DOI | 10.1007/s11010-009-0126-1 |
| Pubmed ID | |
| Authors |
Hana Radilova, Antonin Libra, Sarka Holasova, Martina Safarova, Alena Viskova, Filip Kunc, Martin Buncek |
| Abstract |
Cyclooxygenases are key enzymes in the arachidonic acid metabolism. Their unstable intermediate, prostaglandin H(2), is further metabolized to bioactive lipids by various downstream enzymes. In this study, utilizing short hairpin RNAs, we prepared a cell line of human cervix carcinoma with stable down-regulated cyclooxygenase-1 (COX-1) to assess the impact of COX-1 reduction on the downstream enzymes. We found a significant microsomal prostaglandin E synthase-1 (mPGES-1) suppression. In addition, mRNA expression of multidrug resistance protein 4 (MRP4, ABCC4), supposed to take part in antiviral and anticancer drug transport from cells, was up-regulated after COX-1 down-regulation. Our findings indicate that mPGES-1, believed to be coexpressed preferentially with cyclooxygenase-2, may be coupled to COX-1. ABCC4 up-regulation further supports the assumption of its involvement in prostanoid transport. |
Mendeley readers
The data shown below were compiled from readership statistics for 16 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 6% |
| Unknown | 15 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Lecturer | 3 | 19% |
| Professor > Associate Professor | 3 | 19% |
| Researcher | 3 | 19% |
| Student > Ph. D. Student | 2 | 13% |
| Student > Bachelor | 2 | 13% |
| Other | 2 | 13% |
| Unknown | 1 | 6% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 7 | 44% |
| Neuroscience | 3 | 19% |
| Biochemistry, Genetics and Molecular Biology | 2 | 13% |
| Medicine and Dentistry | 1 | 6% |
| Nursing and Health Professions | 1 | 6% |
| Other | 0 | 0% |
| Unknown | 2 | 13% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 September 2010.
All research outputs
#7,453,827
of 22,787,797 outputs
Outputs from Molecular and Cellular Biochemistry
#417
of 2,301 outputs
Outputs of similar age
#32,505
of 92,860 outputs
Outputs of similar age from Molecular and Cellular Biochemistry
#4
of 15 outputs
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