Title |
In vitro and in vivo characterization of highly purified Human Mesothelioma derived cells
|
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Published in |
BMC Cancer, February 2010
|
DOI | 10.1186/1471-2407-10-54 |
Pubmed ID | |
Authors |
Alice Melotti, Antonio Daga, Daniela Marubbi, Annalisa Zunino, Luciano Mutti, Giorgio Corte |
Abstract |
Malignant pleural mesothelioma is a rare disease known to be resistant to conventional therapies. A better understanding of mesothelioma biology may provide the rationale for new therapeutic strategies. In this regard, tumor cell lines development has been an important tool to study the biological properties of many tumors. However all the cell lines established so far were grown in medium containing at least 10% serum, and it has been shown that primary cell lines cultured under these conditions lose their ability to differentiate, acquire gene expression profiles that differ from that of tissue specific stem cells or the primary tumor they derive from, and in some cases are neither clonogenic nor tumorigenic. Our work was aimed to establish from fresh human pleural mesothelioma samples cell cultures maintaining tumorigenic properties. |
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Geographical breakdown
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