| Title |
RNA interference is required for normal centromere function infission yeast
|
|---|---|
| Published in |
Chromosome Research, February 2003
|
| DOI | 10.1023/a:1022815931524 |
| Pubmed ID | |
| Authors |
Tom Volpe, Vera Schramke, Georgina L. Hamilton, Sharon A. White, Grace Teng, Robert A. Martienssen, Robin C. Allshire |
| Abstract |
In plants, animals and fungi, active centromeres are associated with arrays of repetitive DNA sequences. The outer repeats at fission yeast (Schizosaccharomyces pombe) centromeres are heterochromatic and are required for the assembly of an active centromere. Components of the RNA interference (RNAi) machinery process transcripts derived from these repeats and mediate the formation of silent chromatin. A subfragment of the repeat (dg) is known to induce silencing of marker genes at euchromatic sites and is required for centromere formation. We show that the RNAi components, Argonaute (Ago1), Dicer (Dcr1) and RNA-dependent RNA polymerase (Rdp1), are required to maintain silencing, lysine 9 methylation of histone H3 and association of Swi6 via this dg ectopic silencer. Deletion of Ago1, Dcr1 or Rdp1 disrupts chromosome segregation leading to a high incidence of lagging chromosomes on late anaphase spindles and sensitivity to a microtubule poison. Analysis of dg transcription indicates that csp mutants, previously shown to abrogate centromere silencing and chromosome segregation, are also defective in the regulation of non-coding centromeric RNAs. In addition, histone H3 lysine 9 methylation at, and recruitment of Swi6 and cohesin to, centromeric repeats is disrupted in these mutants. Thus the formation of silent chromatin on dg repeats and the development of a fully functional centromere is dependent on RNAi. |
Mendeley readers
The data shown below were compiled from readership statistics for 170 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 6 | 4% |
| United Kingdom | 3 | 2% |
| Czechia | 2 | 1% |
| Spain | 2 | 1% |
| France | 1 | <1% |
| Netherlands | 1 | <1% |
| China | 1 | <1% |
| Unknown | 154 | 91% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 47 | 28% |
| Student > Ph. D. Student | 42 | 25% |
| Professor > Associate Professor | 18 | 11% |
| Professor | 11 | 6% |
| Student > Master | 11 | 6% |
| Other | 24 | 14% |
| Unknown | 17 | 10% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 98 | 58% |
| Biochemistry, Genetics and Molecular Biology | 44 | 26% |
| Computer Science | 3 | 2% |
| Immunology and Microbiology | 3 | 2% |
| Sports and Recreations | 1 | <1% |
| Other | 4 | 2% |
| Unknown | 17 | 10% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 April 2022.
All research outputs
#8,535,472
of 25,374,647 outputs
Outputs from Chromosome Research
#169
of 536 outputs
Outputs of similar age
#34,234
of 140,949 outputs
Outputs of similar age from Chromosome Research
#1
of 7 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. This one is in the 43rd percentile – i.e., 43% of other outputs scored the same or lower than it.
So far Altmetric has tracked 536 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.1. This one is in the 42nd percentile – i.e., 42% of its peers scored the same or lower than it.
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