| Title |
Electrophoretic mobility of a monotopic membrane protein inserted into the top of supported lipid bilayers
|
|---|---|
| Published in |
The European Physical Journal E, December 2016
|
| DOI | 10.1140/epje/i2016-16127-1 |
| Pubmed ID | |
| Authors |
Frédéric Harb, Marie-Thérèse Giudici-Orticoni, Marianne Guiral, Bernard Tinland |
| Abstract |
We have studied the translational migration of a monotopic membrane protein, the bacterial sulfide quinone reductase (SQR) in supported n-bilayers ([Formula: see text]) under the influence of an electric field parallel to the membrane plane. The direction of the migration changes when the charge of the protein changes its sign. Measuring mobilities at different pH enables us to gain experimental physico-chemical data on SQR as its isoelectric point and its estimated oligomeric state (at least trimeric) when inserted in a lipid membrane. Consequently, in addition to the migration study of membrane proteins in a lipid environment, this experimental system, previously used with a transmembrane protein, is thus suitable to define membrane protein properties in conditions approaching the native ones (in the absence of detergent). |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 7 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 3 | 43% |
| Student > Ph. D. Student | 2 | 29% |
| Professor > Associate Professor | 1 | 14% |
| Unknown | 1 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 3 | 43% |
| Biochemistry, Genetics and Molecular Biology | 2 | 29% |
| Unknown | 2 | 29% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 March 2018.
All research outputs
#15,821,622
of 23,498,099 outputs
Outputs from The European Physical Journal E
#395
of 650 outputs
Outputs of similar age
#259,728
of 423,898 outputs
Outputs of similar age from The European Physical Journal E
#9
of 15 outputs
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