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Combinatorial protein display for the cell-based screening of biomaterials that direct neural stem cell differentiation

Overview of attention for article published in Clinical Materials, November 2006
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Title
Combinatorial protein display for the cell-based screening of biomaterials that direct neural stem cell differentiation
Published in
Clinical Materials, November 2006
DOI 10.1016/j.biomaterials.2006.10.004
Pubmed ID
Authors

Masafumi Nakajima, Toshinari Ishimuro, Koichi Kato, In-Kap Ko, Isao Hirata, Yusuke Arima, Hiroo Iwata

Abstract

Neural stem cell (NSC) has emerged as a potential source for cell replacement therapy following traumatic injuries and degenerative diseases of the central nervous system. However, clinical applications of NSC further require technological advances especially for controlling differentiation of NSC. This study aimed at developing biomaterials that serve to expand undifferentiated NSC or to induce cells with specific phenotypes. Our approach is to construct composite biomaterials that consist of extracellular matrix components and growth factors. In order to optimize matrix-growth factor combinations, we conducted the parallel and rapid screening of composite biomaterials through assays using cell-based arrays. The photo-assisted patterning of an alkanethiol self-assembled monolayer was employed to achieve site-addressable combinatorial immobilization of natural and synthetic matrices incorporated with growth factors including epidermal growth factor (EGF), ciliary neurotrophic factor (CNTF), nerve growth factor (NGF), and neurotrophin-3 (NT-3). NSC obtained from the rat embryonic striatum was cultured directly on the array to screen for cell adhesion, proliferation, and promotion of neuronal and glial specification. The results showed that the significant number of cells adhered to laminin-1, fibronectin, ProNectin, and poly(ethyleneimine). It was found that cells proliferated most extensively on a spot with immobilized EGF among the spots with different matrix-growth factor combinations. The results also showed that neuronal differentiation was promoted on the spots with immobilized NGF or NT-3, and astroglial differentiation with CNTF. Importantly, observed effects of growth factors were frequently altered depending on the type of co-immobilized matrices, suggesting synergic effects of adhesion and growth factor signals.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 120 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Australia 2 2%
United Kingdom 2 2%
United States 2 2%
Japan 2 2%
Canada 1 <1%
Korea, Republic of 1 <1%
Unknown 110 92%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 32 27%
Researcher 25 21%
Student > Master 14 12%
Student > Bachelor 12 10%
Student > Doctoral Student 11 9%
Other 20 17%
Unknown 6 5%
Readers by discipline Count As %
Agricultural and Biological Sciences 29 24%
Engineering 20 17%
Materials Science 19 16%
Chemistry 13 11%
Medicine and Dentistry 11 9%
Other 19 16%
Unknown 9 8%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 March 2015.
All research outputs
#8,616,072
of 25,576,801 outputs
Outputs from Clinical Materials
#4,820
of 10,789 outputs
Outputs of similar age
#31,228
of 90,405 outputs
Outputs of similar age from Clinical Materials
#42
of 75 outputs
Altmetric has tracked 25,576,801 research outputs across all sources so far. This one is in the 43rd percentile – i.e., 43% of other outputs scored the same or lower than it.
So far Altmetric has tracked 10,789 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.9. This one is in the 21st percentile – i.e., 21% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 90,405 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 13th percentile – i.e., 13% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 75 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.