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Mendeley readers
Attention Score in Context
| Title |
The Members of an Epstein-Barr Virus MicroRNA Cluster Cooperate To Transform B Lymphocytes
|
|---|---|
| Published in |
Journal of Virology, July 2011
|
| DOI | 10.1128/jvi.05100-11 |
| Pubmed ID | |
| Authors |
Regina Feederle, Janina Haar, Katharina Bernhardt, Sarah D. Linnstaedt, Helmut Bannert, Helge Lips, Bryan R. Cullen, Henri-Jacques Delecluse |
| Abstract |
Epstein-Barr virus (EBV) transforms B lymphocytes through the expression of the latent viral proteins EBNA and latent membrane protein (LMP). Recently, it has become apparent that microRNAs (miRNAs) also contribute to EBV's oncogenic properties; recombinant EBVs that lack the BHRF1 miRNA cluster display a reduced ability to transform B lymphocytes in vitro. Furthermore, infected cells evince a marked upregulation of the EBNA genes. Using recombinant viruses that lack only one member of the cluster, we now show that all three BHRF1 miRNAs contribute to B-cell transformation. Recombinants that lacked miR-BHRF1-2 or miR-BHRF1-3 displayed enhanced EBNA expression initiated at the Cp and Wp promoters. Interestingly, we find that the deletion of miR-BHRF1-2 reduced the expression level of miR-BHRF1-3 and possibly that of miR-BHRF1-1, demonstrating that the expression of one miRNA can potentiate the expression of other miRNAs located in the same cluster. Therefore, the phenotypic traits of the miR-BHRF1-2 null mutant could result partly from reduced miR-BHRF1-1 and miR-BHRF1-3 expression levels. Nevertheless, using an miR-BHRF1-1 and miR-BHRF1-3 double mutant, we could directly assess and confirm the contribution of miR-BHRF1-2 to B-cell transformation. Furthermore, we found that the potentiating effect of miR-BHRF1-2 on miR-BHRF1-3 synthesis can be reproduced with simple expression plasmids, provided that both miRNAs are processed from the same transcript. Therefore, this enhancing effect does not result from an idiosyncrasy of the EBV genome but rather reflects a general property of these miRNAs. This study highlights the advantages of arranging the BHRF1 miRNAs in clusters: it allows the synchronous and synergistic expression of genetic elements that cooperate to transform their target cells. |
Mendeley readers
The data shown below were compiled from readership statistics for 73 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 1% |
| India | 1 | 1% |
| Netherlands | 1 | 1% |
| France | 1 | 1% |
| Unknown | 69 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 16 | 22% |
| Researcher | 14 | 19% |
| Student > Bachelor | 12 | 16% |
| Student > Master | 9 | 12% |
| Professor > Associate Professor | 6 | 8% |
| Other | 6 | 8% |
| Unknown | 10 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 34 | 47% |
| Biochemistry, Genetics and Molecular Biology | 11 | 15% |
| Medicine and Dentistry | 6 | 8% |
| Immunology and Microbiology | 4 | 5% |
| Chemistry | 2 | 3% |
| Other | 4 | 5% |
| Unknown | 12 | 16% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 September 2011.
All research outputs
#8,534,528
of 25,373,627 outputs
Outputs from Journal of Virology
#11,220
of 25,691 outputs
Outputs of similar age
#46,664
of 128,344 outputs
Outputs of similar age from Journal of Virology
#90
of 206 outputs
Altmetric has tracked 25,373,627 research outputs across all sources so far. This one is in the 43rd percentile – i.e., 43% of other outputs scored the same or lower than it.
So far Altmetric has tracked 25,691 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 7.5. This one is in the 20th percentile – i.e., 20% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 128,344 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 34th percentile – i.e., 34% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 206 others from the same source and published within six weeks on either side of this one. This one is in the 28th percentile – i.e., 28% of its contemporaries scored the same or lower than it.