| Title |
Transplantation of reconstructed human skin on nude mice: a model system to study expression of human tenascin-X and elastic fiber components
|
|---|---|
| Published in |
Cell and Tissue Research, November 2004
|
| DOI | 10.1007/s00441-004-1011-6 |
| Pubmed ID | |
| Authors |
Manon C. Zweers, Joost Schalkwijk, Toin H. van Kuppevelt, Ivonne M. van Vlijmen-Willems, Mieke Bergers, Claire Lethias, Evert N. Lamme |
| Abstract |
Tenascin-X is a large extracellular matrix protein that is widely expressed in connective tissues during development and in the adult. Genetically determined deficiency of tenascin-X causes the connective tissue disease Ehlers-Danlos syndrome. These patients show reduced collagen density and fragmentation of elastic fibers in their skin. In vitro studies on the role of tenascin-X in elastic fiber biology are hampered because monolayers of fibroblasts do not deposit tenascin-X and elastic fibers into the extracellular matrix. Here, we applied an organotypic culture model of fibroblasts and keratinocytes to address this issue. We investigated the deposition of tenascin-X and elastin into skin-equivalent in vitro and also in vivo after transplantation onto immunodeficient mice. Whereas tenascin-C and fibrillin-1 were readily expressed in the skin-equivalents before transplantation, tenascin-X and elastin were not present. Three weeks post-grafting, a network of elastin was observed that coincided with the appearance of tenascin-X. At the ultrastructural level, microfibrils were observed, some of which were associated with elastin. Transplanted skin-equivalents containing tenascin-X-deficient fibroblasts showed deposition of immunoreactive elastin in similar quantities and distribution as those containing control fibroblasts. This suggests that tenascin-X is important for the stability and maintenance of established elastin fibers, rather than for the initial phase of elastogenesis. Thus, the transplantation of reconstructed skin on nude mice allows the study of tenascin-X and elastin expression and could be used as a model system to study the potential role of tenascin-X in matrix assembly and stability. |
Mendeley readers
The data shown below were compiled from readership statistics for 14 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 2 | 14% |
| United States | 1 | 7% |
| Netherlands | 1 | 7% |
| Unknown | 10 | 71% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 5 | 36% |
| Other | 4 | 29% |
| Researcher | 2 | 14% |
| Student > Master | 1 | 7% |
| Student > Bachelor | 1 | 7% |
| Other | 0 | 0% |
| Unknown | 1 | 7% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 4 | 29% |
| Agricultural and Biological Sciences | 4 | 29% |
| Biochemistry, Genetics and Molecular Biology | 2 | 14% |
| Arts and Humanities | 1 | 7% |
| Social Sciences | 1 | 7% |
| Other | 1 | 7% |
| Unknown | 1 | 7% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 December 2017.
All research outputs
#7,862,539
of 23,839,820 outputs
Outputs from Cell and Tissue Research
#527
of 2,279 outputs
Outputs of similar age
#37,321
of 144,267 outputs
Outputs of similar age from Cell and Tissue Research
#3
of 7 outputs
Altmetric has tracked 23,839,820 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,279 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 45th percentile – i.e., 45% of its peers scored the same or lower than it.
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