| Title |
Differential expression of interferon-induced microRNAs in patients with chronic hepatitis C virus infection treated with pegylated interferon alpha
|
|---|---|
| Published in |
Virology Journal, November 2010
|
| DOI | 10.1186/1743-422x-7-311 |
| Pubmed ID | |
| Authors |
Carolina Scagnolari, Pompea Zingariello, Jacopo Vecchiet, Carla Selvaggi, Delia Racciatti, Gloria Taliani, Elisabetta Riva, Eligio Pizzigallo, Guido Antonelli |
| Abstract |
There have been reports of in-vitro interferon (IFN)-mediated antiviral activity against the hepatitis C virus (HCV) through microRNAs (miRNAs). The main aim of this study was to evaluate the expression of several miRNAs (miR-1, miR-30, miR-128, miR-196, miR-296) in peripheral blood mononuclear cells (PBMCs) from healthy individuals after in vitro IFN-treatment and in PBMCs from patients with chronic hepatitis C (CHC) before and 12 hours after the first injection of pegylated IFN alpha. We demonstrated that expression of these miRNAs could be recorded in PBMCs collected from healthy individuals before and after in-vitro IFN alpha treatment. Our analysis revealed that the levels of expression of all miRNAs investigated in patients with CHC were different to those in healthy individuals. When levels of the miRNAs were measured 12 hours after the first IFN injection, increases in expression levels of IFN-induced miRNAs were observed in 25-50% of patients, depending on the type of miRNA examined. No correlations were observed between HCV viral load, alanine aminotransferase status and expression of miRNA. Together these findings suggest that: (i) IFN alpha in-vitro treatment of PBMCs leads to a transcriptional induction of all miRNAs investigated; (ii) miRNAs can be induced differentially by IFN treatment in patients with HCV. Given the importance of miRNAs in defending the host against virus infections, it is possible that IFN-induced miRNAs may represent an important determinant of the clinical outcome of IFN therapy in HCV infection. |
Mendeley readers
The data shown below were compiled from readership statistics for 43 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Russia | 1 | 2% |
| Unknown | 42 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 16 | 37% |
| Student > Ph. D. Student | 5 | 12% |
| Professor > Associate Professor | 5 | 12% |
| Student > Master | 3 | 7% |
| Student > Bachelor | 2 | 5% |
| Other | 8 | 19% |
| Unknown | 4 | 9% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 18 | 42% |
| Medicine and Dentistry | 8 | 19% |
| Immunology and Microbiology | 5 | 12% |
| Biochemistry, Genetics and Molecular Biology | 4 | 9% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 5% |
| Other | 0 | 0% |
| Unknown | 6 | 14% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 July 2011.
All research outputs
#7,453,827
of 22,787,797 outputs
Outputs from Virology Journal
#898
of 3,042 outputs
Outputs of similar age
#36,076
of 100,599 outputs
Outputs of similar age from Virology Journal
#16
of 38 outputs
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