| Title |
Biocompatibility of Tygon® tubing in microfluidic cell culture
|
|---|---|
| Published in |
Biomedical Microdevices, February 2015
|
| DOI | 10.1007/s10544-015-9938-9 |
| Pubmed ID | |
| Authors |
Xiao Jiang, Rex E. Jeffries, Miguel A. Acosta, Andrey P. Tikunov, Jeffrey M. Macdonald, Glenn M. Walker, Michael P. Gamcsik |
| Abstract |
Growth of the MDA-MB-231 breast cancer cell line in microfluidic channels was inhibited when culture media was delivered to the channels via microbore Tygon® tubing. Culture media incubated within this tubing also inhibited growth of these cells in conventional 96-well plates. These detrimental effects were not due to depletion of critical nutrients due to adsorption of media components onto the tubing surface. A pH change was also ruled out as a cause. Nuclear magnetic resonance spectroscopy of the cell growth media before and after incubation in the tubing confirmed no detectable loss of media components but did detect the presence of additional unidentified signals in the aliphatic region of the spectrum. These results indicate leaching of a chemical species from microbore Tygon® tubing that can affect cell growth in microfluidic devices. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 23 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Israel | 1 | 4% |
| Netherlands | 1 | 4% |
| Unknown | 21 | 91% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 9 | 39% |
| Student > Ph. D. Student | 4 | 17% |
| Student > Bachelor | 4 | 17% |
| Student > Master | 2 | 9% |
| Professor > Associate Professor | 1 | 4% |
| Other | 0 | 0% |
| Unknown | 3 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Engineering | 6 | 26% |
| Biochemistry, Genetics and Molecular Biology | 4 | 17% |
| Chemistry | 3 | 13% |
| Agricultural and Biological Sciences | 2 | 9% |
| Medicine and Dentistry | 1 | 4% |
| Other | 2 | 9% |
| Unknown | 5 | 22% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 February 2015.
All research outputs
#15,321,186
of 22,787,797 outputs
Outputs from Biomedical Microdevices
#554
of 747 outputs
Outputs of similar age
#209,632
of 352,275 outputs
Outputs of similar age from Biomedical Microdevices
#11
of 22 outputs
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So far Altmetric has tracked 747 research outputs from this source. They receive a mean Attention Score of 4.1. This one is in the 20th percentile – i.e., 20% of its peers scored the same or lower than it.
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We're also able to compare this research output to 22 others from the same source and published within six weeks on either side of this one. This one is in the 36th percentile – i.e., 36% of its contemporaries scored the same or lower than it.