| Title |
An improved physical and genetic map of the genome of alkaliphilic Bacillus sp. C-125
|
|---|---|
| Published in |
Extremophiles, January 1999
|
| DOI | 10.1007/s007920050095 |
| Pubmed ID | |
| Authors |
H. Takami, Kaoru Nakasone, Chie Hirama, Yoshihiro Takaki, Noriaki Masui, Fumie Fuji, Yuka Nakamura, Akira Inoue |
| Abstract |
Among alkaliphilic bacteria reported so far, Bacillus sp. C-125 is the strain most thoroughly characterized physiologically, biochemically, and genetically. A physical map of the chromosome of this strain was constructed to facilitate further genome analysis, and the genome size was revised from 3.7 to 4.25Mb. Complete digestion of the chromosomal DNA with two rare cut restriction endonucleases, AscI and Sse8387I, each yielded 20 fragments ranging in size from 20 to 600 kb. Seventeen linking clones were isolated in each instance to join the adjacent AscI or Sse8387I fragments in the chromosomal map. All AscI linking clones isolated were sequenced and analyzed by comparison with the BSORF database to map the genes in the chromosome of strain C-125. Several ORFs showing significant similarities to those of B. subtilis in the AscI linking clones were positioned on the physical map. The oriC region of the C-125 chromosome was identified by southern blot analysis with a DNA probe containing the gyrB region. |
Mendeley readers
The data shown below were compiled from readership statistics for 18 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 1 | 6% |
| France | 1 | 6% |
| Unknown | 16 | 89% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 4 | 22% |
| Professor | 3 | 17% |
| Student > Master | 3 | 17% |
| Student > Ph. D. Student | 2 | 11% |
| Student > Bachelor | 1 | 6% |
| Other | 2 | 11% |
| Unknown | 3 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 8 | 44% |
| Biochemistry, Genetics and Molecular Biology | 3 | 17% |
| Earth and Planetary Sciences | 1 | 6% |
| Medicine and Dentistry | 1 | 6% |
| Engineering | 1 | 6% |
| Other | 0 | 0% |
| Unknown | 4 | 22% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 31 October 2014.
All research outputs
#8,535,472
of 25,374,647 outputs
Outputs from Extremophiles
#235
of 816 outputs
Outputs of similar age
#24,657
of 109,586 outputs
Outputs of similar age from Extremophiles
#3
of 5 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. This one is in the 43rd percentile – i.e., 43% of other outputs scored the same or lower than it.
So far Altmetric has tracked 816 research outputs from this source. They receive a mean Attention Score of 4.3. This one is in the 41st percentile – i.e., 41% of its peers scored the same or lower than it.
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