| Title |
Efficient isolation of highly purified tonsil B lymphocytes using RosetteSep with allogeneic human red blood cells
|
|---|---|
| Published in |
BMC Immunology, May 2009
|
| DOI | 10.1186/1471-2172-10-30 |
| Pubmed ID | |
| Authors |
Jonathan Zuccolo, Tammy L Unruh, Julie P Deans |
| Abstract |
Human tonsils are a rich source of B lymphocytes exhibiting a variety of phenotypes and activation states. Existing methods of purification are time consuming or costly. The aim of the present study was to optimize conditions to isolate large numbers of highly purified primary B lymphocytes from tonsils in a short and cost-effective single step, using a commercially available reagent designed for purifying cells from whole blood (RosetteSep). This technique relies on the presence of the large excess of red blood cells in whole blood for the formation of immunorosettes, whereas single cell suspensions from tonsils contain relatively few red blood cells. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 16 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 6% |
| Unknown | 15 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 6 | 38% |
| Researcher | 4 | 25% |
| Student > Master | 2 | 13% |
| Student > Bachelor | 1 | 6% |
| Unspecified | 1 | 6% |
| Other | 2 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 8 | 50% |
| Biochemistry, Genetics and Molecular Biology | 2 | 13% |
| Unspecified | 1 | 6% |
| Computer Science | 1 | 6% |
| Immunology and Microbiology | 1 | 6% |
| Other | 2 | 13% |
| Unknown | 1 | 6% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 January 2020.
All research outputs
#6,413,742
of 22,787,797 outputs
Outputs from BMC Immunology
#105
of 586 outputs
Outputs of similar age
#33,404
of 111,865 outputs
Outputs of similar age from BMC Immunology
#2
of 5 outputs
Altmetric has tracked 22,787,797 research outputs across all sources so far. This one has received more attention than most of these and is in the 70th percentile.
So far Altmetric has tracked 586 research outputs from this source. They receive a mean Attention Score of 3.7. This one has done well, scoring higher than 81% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 111,865 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 68% of its contemporaries.
We're also able to compare this research output to 5 others from the same source and published within six weeks on either side of this one. This one has scored higher than 3 of them.