| Title |
Truncated form of tenascin-X, XB-S, interacts with mitotic motor kinesin Eg5
|
|---|---|
| Published in |
Molecular and Cellular Biochemistry, August 2008
|
| DOI | 10.1007/s11010-008-9898-y |
| Pubmed ID | |
| Authors |
Toshiya Endo, Hiroyoshi Ariga, Ken-ichi Matsumoto |
| Abstract |
XB-S is a protein with an amino-terminal-truncated form of tenascin-X (TNXB). However, the precise roles of XB-S in vivo are unknown. In this study, to determine the role of XB-S in vivo, we screened XB-S-binding proteins. FLAG-tagged XB-S was transiently introduced into 293T cells. Then its associated proteins were purified by immunoprecipitation using an anti-FLAG antibody and its components were identified by mass spectrometric analyses. Mitotic motor kinesin Eg5 was identified in the immunoprecipitates. XB-S and Eg5 proteins were co-localized in the cytoplasm in interphase and mitosis, but XB-S did not localize on mitotic spindle microtubules, on which Eg5 prominently localized in mitosis. As for Eg5 binding to XB-S, glutathione S-transferase-fused XB-S expressed in vitro directly bound to full-length Eg5 translated in reticulocyte lysate, and the XB-S-binding region was located in the motor domain of Eg5. Furthermore, during cell cycle progression XB-S showed a similar expression profile to that of Eg5. These results suggest possible involvement of XB-S in the function of Eg5. |
Mendeley readers
The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 9% |
| United States | 1 | 9% |
| Unknown | 9 | 82% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 5 | 45% |
| Student > Ph. D. Student | 2 | 18% |
| Other | 2 | 18% |
| Student > Doctoral Student | 1 | 9% |
| Unknown | 1 | 9% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 6 | 55% |
| Biochemistry, Genetics and Molecular Biology | 2 | 18% |
| Computer Science | 1 | 9% |
| Medicine and Dentistry | 1 | 9% |
| Unknown | 1 | 9% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 December 2017.
All research outputs
#7,453,827
of 22,787,797 outputs
Outputs from Molecular and Cellular Biochemistry
#417
of 2,301 outputs
Outputs of similar age
#29,077
of 82,433 outputs
Outputs of similar age from Molecular and Cellular Biochemistry
#7
of 16 outputs
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