| Title |
Chromosome structure: improved immunolabeling for electron microscopy
|
|---|---|
| Published in |
Chromosoma, September 2005
|
| DOI | 10.1007/s00412-005-0023-7 |
| Pubmed ID | |
| Authors |
Kazuhiro Maeshima, Michail Eltsov, Ulrich K. Laemmli |
| Abstract |
To structurally dissect mitotic chromosomes, we aim to position along the folded chromatin fiber proteins involved in long-range order, such as topoisomerase IIalpha (topoIIalpha) and condensin. Immuno-electron microscopy (EM) of thin-sectioned chromosomes is the method of choice toward this goal. A much-improved immunoprocedure that avoids problems associated with aldehyde fixation, such as chemical translinking and networking of chromatin fibers, is reported here. We show that ultraviolet irradiation of isolated nuclei or chromosomes facilitates high-level specific immunostaining, as established by fluorescence microscopy with a variety of antibodies and especially by immuno-EM. Ultrastructural localizations of topoIIalpha and condensin I component hBarren (hBar; hCAP-H) in mitotic chromosomes were studied by immuno-EM. We show that the micrographs of thin-sectioned chromosomes map topoIIalpha and hBar to the center of the chromosomal body where the chromatin fibers generally converge. This localization is defined by many clustered gold particles with only rare individual particles in the peripheral halo. The data obtained are consistent with the view that condensin and perhaps topoIIalpha tether chromatin to loops according to a scaffolding-type model. |
Mendeley readers
The data shown below were compiled from readership statistics for 86 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | 2% |
| Brazil | 2 | 2% |
| France | 1 | 1% |
| Canada | 1 | 1% |
| United Kingdom | 1 | 1% |
| Unknown | 79 | 92% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 24 | 28% |
| Student > Ph. D. Student | 19 | 22% |
| Professor | 10 | 12% |
| Student > Master | 9 | 10% |
| Student > Doctoral Student | 4 | 5% |
| Other | 12 | 14% |
| Unknown | 8 | 9% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 40 | 47% |
| Biochemistry, Genetics and Molecular Biology | 22 | 26% |
| Chemistry | 4 | 5% |
| Physics and Astronomy | 4 | 5% |
| Environmental Science | 1 | 1% |
| Other | 4 | 5% |
| Unknown | 11 | 13% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 April 2013.
All research outputs
#7,453,827
of 22,787,797 outputs
Outputs from Chromosoma
#178
of 757 outputs
Outputs of similar age
#20,520
of 59,120 outputs
Outputs of similar age from Chromosoma
#2
of 6 outputs
Altmetric has tracked 22,787,797 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.
So far Altmetric has tracked 757 research outputs from this source. They receive a mean Attention Score of 3.7. This one is in the 34th percentile – i.e., 34% of its peers scored the same or lower than it.
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We're also able to compare this research output to 6 others from the same source and published within six weeks on either side of this one. This one has scored higher than 4 of them.