| Title |
Mapping and expression studies of the mir17-92 cluster on pig Chromosome 11
|
|---|---|
| Published in |
Mammalian Genome, September 2005
|
| DOI | 10.1007/s00335-005-0013-3 |
| Pubmed ID | |
| Authors |
Milena Sawera, Jan Gorodkin, Susanna Cirera, Merete Fredholm |
| Abstract |
We have identified the first porcine microRNA (miRNA) cluster (the mir17-92 cluster) and localized it to the q-arm of pig Chromosome 11. The miRNA cluster was found by sequence similarity search with human miRNA sequences against the pig genomic data generated within the Sino-Danish pig genome project. The resulting data contained three complete and two incomplete miRNA precursors of seven miRNAs from the human mir17-92 cluster. Because there is a 100% sequence identity between the four pig miRNAs and the corresponding human miRNAs, the sequences of three unavailable pig miRNAs were derived from the human data. The expression profiles of seven studied miRNAs were analyzed by hybridization to Northern blots containing five porcine tissues: cerebellum, cortex, hippocampus, kidney, and liver. In order to determine the localization of the mir17-92 cluster in the pig genome, we mapped it by PCR in the porcine somatic cell hybrid (SCH) panel and in the INRA-University of Minnesota (INRA-UMN) porcine radiation hybrid (IMpRH) panel. The PCR results enabled us to localize this cluster to the q-arm of pig Chromosome 11 and map it in relation to two microsatellites. Our study presents the first expression analyses of miRNAs in pig and adds information for further functional studies in this species. |
Mendeley readers
The data shown below were compiled from readership statistics for 26 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 3 | 12% |
| Spain | 1 | 4% |
| China | 1 | 4% |
| Unknown | 21 | 81% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 12 | 46% |
| Student > Ph. D. Student | 4 | 15% |
| Student > Doctoral Student | 2 | 8% |
| Professor | 2 | 8% |
| Student > Bachelor | 2 | 8% |
| Other | 3 | 12% |
| Unknown | 1 | 4% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 10 | 38% |
| Biochemistry, Genetics and Molecular Biology | 7 | 27% |
| Computer Science | 4 | 15% |
| Veterinary Science and Veterinary Medicine | 1 | 4% |
| Medicine and Dentistry | 1 | 4% |
| Other | 0 | 0% |
| Unknown | 3 | 12% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 November 2010.
All research outputs
#7,453,827
of 22,787,797 outputs
Outputs from Mammalian Genome
#318
of 1,126 outputs
Outputs of similar age
#20,482
of 58,686 outputs
Outputs of similar age from Mammalian Genome
#2
of 3 outputs
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