| Title |
Heterochromatin protein HP1Hsβ(p25β) and its localization with centromeres in mitosis
|
|---|---|
| Published in |
Chromosoma, June 1997
|
| DOI | 10.1007/s004120050219 |
| Pubmed ID | |
| Authors |
Kiyoshi Furuta, Edward K. L. Chan, Kendo Kiyosawa, Georg Reimer, Christoph Luderschmidt, Eng M. Tan |
| Abstract |
Some autoimmune sera containing anticentromere autoantibodies also recognize a doublet of Mr 23 000 (p23) and 25 000 (p25) in addition to CENP (centromere protein)-A (Mr 19 000), -B (Mr 80 000), and -C (Mr 140 000). A p25 antigen (HP1(Hsalpha)) has been shown to be a human homolog of Drosophila HP1 (heterochromatin protein 1). We have isolated a cDNA clone encoding another form of p25 (HP1(Hsbeta ) or p25beta) from a lambdaZap HepG2 library using human autoimmune serum. The deduced amino acid sequence of the clone contained a conserved chromodomain (chromatin modifier domain) in the N-terminal region and a heterochromatin binding domain in the C-terminal region. In immunofluorescence experiments, only affinity purified antibodies reactive with the C-terminal (amino acids 70-185) domain showed nucleoplasmic and heterochromatin staining, whereas N-terminal (amino acids 1-115) specific antibodies were nonreactive. In metaphase chromosome spreads, the C-terminal domain antibody was also localized to the centromeric regions of chromosomes. Association with centromeres was most prominent at anaphase and changed to a more generalized association with whole chromosomes in telophase. The cooccurrence of autoantibodies to centromere proteins and HP1 in certain autoimmune diseases might be a reflection of coordinated immune responses to these closely associated sets of proteins. |
Mendeley readers
The data shown below were compiled from readership statistics for 24 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Italy | 1 | 4% |
| Germany | 1 | 4% |
| Unknown | 22 | 92% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 7 | 29% |
| Researcher | 5 | 21% |
| Professor | 4 | 17% |
| Other | 2 | 8% |
| Student > Master | 2 | 8% |
| Other | 2 | 8% |
| Unknown | 2 | 8% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 9 | 38% |
| Biochemistry, Genetics and Molecular Biology | 6 | 25% |
| Immunology and Microbiology | 3 | 13% |
| Medicine and Dentistry | 3 | 13% |
| Unknown | 3 | 13% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 March 2020.
All research outputs
#8,535,472
of 25,374,647 outputs
Outputs from Chromosoma
#206
of 786 outputs
Outputs of similar age
#9,449
of 29,200 outputs
Outputs of similar age from Chromosoma
#5
of 9 outputs
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