| Title |
Substrate specificity of N-methyltransferase involved in purine alkaloids synthesis is dependent upon one amino acid residue of the enzyme
|
|---|---|
| Published in |
Molecular Genetics and Genomics, December 2005
|
| DOI | 10.1007/s00438-005-0070-z |
| Pubmed ID | |
| Authors |
Naho Yoneyama, Hanayo Morimoto, Chuang-Xing Ye, Hiroshi Ashihara, Kouichi Mizuno, Misako Kato |
| Abstract |
Caffeine (1,3,7-trimethylxanthine) and theobromine (3,7-dimethylxanthine) are the major purine alkaloids in plants. To investigate the diversity of N-methyltransferases involved in purine alkaloid biosynthesis, we isolated the genes homologous for caffeine synthase from theobromine-accumulating plants. The predicted amino acid sequences of N-methyltransferases in theobromine-accumulating species in Camellia were more than 80% identical to caffeine synthase in C. sinensis. However, there was a little homology among the N-methyltransferases between Camellia and Theobroma. The recombinant enzymes derived from theobromine-accumulating plants had only 3-N-methyltransferase activity. The accumulation of purine alkaloids was, therefore, dependent on the substrate specificity of N-methyltransferase determined by one amino acid residue in the central part of the protein. |
Mendeley readers
The data shown below were compiled from readership statistics for 52 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Brazil | 2 | 4% |
| Belgium | 1 | 2% |
| Unknown | 49 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 13 | 25% |
| Researcher | 9 | 17% |
| Student > Master | 6 | 12% |
| Professor | 5 | 10% |
| Student > Bachelor | 4 | 8% |
| Other | 6 | 12% |
| Unknown | 9 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 26 | 50% |
| Biochemistry, Genetics and Molecular Biology | 9 | 17% |
| Chemistry | 5 | 10% |
| Computer Science | 1 | 2% |
| Unknown | 11 | 21% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 April 2014.
All research outputs
#8,534,528
of 25,371,288 outputs
Outputs from Molecular Genetics and Genomics
#920
of 3,318 outputs
Outputs of similar age
#42,298
of 162,824 outputs
Outputs of similar age from Molecular Genetics and Genomics
#2
of 8 outputs
Altmetric has tracked 25,371,288 research outputs across all sources so far. This one is in the 43rd percentile – i.e., 43% of other outputs scored the same or lower than it.
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