| Title |
Expression of (cac)n/(gtg)n simple repetitive sequences in mRNA of human lymphocytes
|
|---|---|
| Published in |
Human Genetics, January 1994
|
| DOI | 10.1007/bf00218910 |
| Pubmed ID | |
| Authors |
Cornelia Epplen, Jörg T. Epplen |
| Abstract |
Di- and trinucleotide tandem repeat sequences are ubiquitously interspersed and are often polymorphic in the human genome. We have analyzed the transcription of simple (cac)n/(gtg)n repeats in the cDNA of RNA from human lymphocytes. When using such motifs as probes in RNA hybridization experiments, distinct signals are scarcely demonstrable. In order to investigate mRNA sequences that contain such simple repeats, 1 million phage clones from cDNA libraries were screened with the probe (CAC)5. From 50 hybridizing phages, 38 clones were successfully isolated and characterized. The lengths of the transcripts ranged from 120 bp to 3.5 kb. More than 15 different additional simple repeat motifs were found immediately next to or distant to the (cac)n/(gtg)n repeat. In 12 clones, significant homologies were identified with a wide variety of unrelated genes, such as a processed pseudogene of human ubiquitin, serin protease inhibitor genes, a gene candidate from yeast, and sequence-tagged sites of man and mouse. Of the clones, 18% represented mRNA of MHC class I promotor binding protein; 79% displayed novel single copy sequences or partial similarity to many different organelle and nuclear genomes of animal, fungal, bacterial, and viral sequences. These data indicate that short (cac)n/(gtg)n stretches (n < or = 6) are sometimes contained in open reading frames, but more often in the 3' and 5' untranslated portions of mature mRNAs. Longer stretches of perfect simple (cac)n/(gtg)n repeats can rarely be recovered, even from the hnRNA of human lymphocytes. |
Mendeley readers
The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 3 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Professor | 1 | 33% |
| Researcher | 1 | 33% |
| Other | 1 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Environmental Science | 1 | 33% |
| Agricultural and Biological Sciences | 1 | 33% |
| Unknown | 1 | 33% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 April 2023.
All research outputs
#7,729,343
of 23,505,669 outputs
Outputs from Human Genetics
#960
of 2,996 outputs
Outputs of similar age
#14,676
of 72,257 outputs
Outputs of similar age from Human Genetics
#4
of 17 outputs
Altmetric has tracked 23,505,669 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,996 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.4. This one is in the 21st percentile – i.e., 21% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 72,257 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 21st percentile – i.e., 21% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 17 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.