| Title |
Optimisation of sodium and potassium concentrations and pH in the artificial seminal plasma of common carp Cyprinus carpio L.
|
|---|---|
| Published in |
Fish Physiology and Biochemistry, March 2018
|
| DOI | 10.1007/s10695-018-0491-3 |
| Pubmed ID | |
| Authors |
Beata Irena Cejko, Ákos Horváth, Timea Kollár, Eszter Kása, Jelena Lujić, Zoran Marinović, Béla Urbányi, Radosław Kajetan Kowalski |
| Abstract |
The effect of sodium and potassium concentrations as well as optimal pH on the motility of common carp Cyprinus carpio L. sperm during short-term storage in artificial seminal plasma (ASP) was investigated. Sperm was collected from individual males (n = 5) and each sample diluted tenfold (1:9) in ASP (sperm:extender) containing 2 mM CaCl2, 1 mM Mg2SO4 and 20 mM Tris at pH 8.0 and supplemented by the following concentrations of sodium and potassium (mM/mM): 0/150, 20/130, 40/110, 75/75, 110/40, 130/20 and 150/0. The osmolality of all ASP variants was set at 310 mOsm kg-1. Sperm motility was measured using a CASA system during 72 h of storage. Immediately after dilution, sperm motility was high (90%) both in each variant and in the control group (fresh sperm). After 72-h storage, the highest sperm motility was noted in ASP containing 110 mM NaCl and 40 mM KCl. No differences were found in the motility of samples preserved within the pH range of 7.0-9.0. Our data suggest that for the short-term storage of common carp sperm, whereas the pH of the solution does not play a crucial role, a specific potassium concentration of around 40 mM is required. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 39 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 39 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 7 | 18% |
| Researcher | 5 | 13% |
| Student > Doctoral Student | 4 | 10% |
| Student > Postgraduate | 4 | 10% |
| Professor > Associate Professor | 3 | 8% |
| Other | 8 | 21% |
| Unknown | 8 | 21% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 18 | 46% |
| Unspecified | 2 | 5% |
| Biochemistry, Genetics and Molecular Biology | 2 | 5% |
| Engineering | 2 | 5% |
| Medicine and Dentistry | 2 | 5% |
| Other | 2 | 5% |
| Unknown | 11 | 28% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 March 2018.
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#20,469,520
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Outputs from Fish Physiology and Biochemistry
#608
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#293,521
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Outputs of similar age from Fish Physiology and Biochemistry
#18
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