| Title |
Transcriptome analysis provides insights into the delayed sticky disease symptoms in Carica papaya
|
|---|---|
| Published in |
Plant Cell Reports, March 2018
|
| DOI | 10.1007/s00299-018-2281-x |
| Pubmed ID | |
| Authors |
Johana Madroñero, Silas P. Rodrigues, Tathiana F. S. Antunes, Paolla M. V. Abreu, José A. Ventura, A. Alberto R. Fernandes, Patricia Machado Bueno Fernandes |
| Abstract |
Global gene expression analysis indicates host stress responses, mainly those mediated by SA, associated to the tolerance to sticky disease symptoms at pre-flowering stage in Carica papaya. Carica papaya plants develop the papaya sticky disease (PSD) as a result of the combined infection of papaya meleira virus (PMeV) and papaya meleira virus 2 (PMeV2), or PMeV complex. PSD symptoms appear only after C. papaya flowers. To understand the mechanisms involved in this phenomenon, the global gene expression patterns of PMeV complex-infected C. papaya at pre-and post-flowering stages were assessed by RNA-Seq. The result was 633 and 88 differentially expressed genes at pre- and post-flowering stages, respectively. At pre-flowering stage, genes related to stress and transport were up-regulated while metabolism-related genes were down-regulated. It was observed that induction of several salicylic acid (SA)-activated genes, including PR1, PR2, PR5, WRKY transcription factors, ROS and callose genes, suggesting SA signaling involvement in the delayed symptoms. In fact, pre-flowering C. papaya treated with exogenous SA showed a tendency to decrease the PMeV and PMeV2 loads when compared to control plants. However, pre-flowering C. papaya also accumulated transcripts encoding a NPR1-inhibitor (NPR1-I/NIM1-I) candidate, genes coding for UDP-glucosyltransferases (UGTs) and several genes involved with ethylene pathway, known to be negative regulators of SA signaling. At post-flowering, when PSD symptoms appeared, the down-regulation of PR-1 encoding gene and the induction of BSMT1 and JA metabolism-related genes were observed. Hence, SA signaling likely operates at the pre-flowering stage of PMeV complex-infected C. papaya inhibiting the development of PSD symptoms, but the induction of its negative regulators prevents the full-scale and long-lasting tolerance. |
X Demographics
The data shown below were collected from the profiles of 11 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| India | 2 | 18% |
| Mexico | 1 | 9% |
| Netherlands | 1 | 9% |
| Brazil | 1 | 9% |
| Ireland | 1 | 9% |
| Colombia | 1 | 9% |
| Unknown | 4 | 36% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 5 | 45% |
| Members of the public | 5 | 45% |
| Science communicators (journalists, bloggers, editors) | 1 | 9% |
Mendeley readers
The data shown below were compiled from readership statistics for 40 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 40 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 9 | 23% |
| Student > Master | 6 | 15% |
| Student > Doctoral Student | 4 | 10% |
| Other | 3 | 8% |
| Student > Bachelor | 2 | 5% |
| Other | 5 | 13% |
| Unknown | 11 | 28% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 15 | 38% |
| Agricultural and Biological Sciences | 9 | 23% |
| Computer Science | 2 | 5% |
| Veterinary Science and Veterinary Medicine | 1 | 3% |
| Medicine and Dentistry | 1 | 3% |
| Other | 0 | 0% |
| Unknown | 12 | 30% |
Attention Score in Context
This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 February 2020.
All research outputs
#4,970,370
of 24,780,938 outputs
Outputs from Plant Cell Reports
#310
of 2,322 outputs
Outputs of similar age
#90,446
of 337,370 outputs
Outputs of similar age from Plant Cell Reports
#11
of 27 outputs
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