| Title |
Characterization of the membranous denitrification enzymes nitrite reductase (cytochrome cd1) and copper-containing nitrous oxide reductase from Thiobacillus denitrificans
|
|---|---|
| Published in |
Archives of Microbiology, January 1996
|
| DOI | 10.1007/s002030050296 |
| Pubmed ID | |
| Authors |
U. H. Hole, Kai-Uwe Vollack, Walter G. Zumft, Effi Eisenmann, Roman A. Siddiqui, Bärbel Friedrich, Peter M. H. Kroneck |
| Abstract |
Cytochrome cd1-nitrite reductase and nitrous oxide reductase of Thiobacillus denitrificans were purified and characterized by biochemical and immunochemical methods. In contrast to the generally soluble nature of the denitrification enzymes, these two enzymes were isolated from the membrane fraction of T. denitrificans and remained active after solubilization with Triton X-100. The properties of the membrane-derived enzymes were similar to those of their soluble counterparts from the same organism. Nitrous oxide reductase activity was inhibited by acetylene. Nitrite reductase and nitrous oxide reductase cross-reacted with antisera raised against the soluble enzymes from Pseudomonas stutzeri. The nirS, norBC, and nosZ genes encoding the cytochrome cd1-nitrite reductase, nitric oxide reductase, and nitrous oxide reductase, respectively, from P. stutzeri hybridized with genomic DNA from T. denitrificans. Cross-reactivity and similar N-terminal amino acid and gene sequences suggest that the primary structures of the Thiobacillus enzymes are homologous to the soluble proteins from P. stutzeri. |
Mendeley readers
The data shown below were compiled from readership statistics for 43 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 2% |
| Unknown | 42 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 10 | 23% |
| Researcher | 10 | 23% |
| Student > Master | 7 | 16% |
| Professor | 4 | 9% |
| Student > Bachelor | 2 | 5% |
| Other | 5 | 12% |
| Unknown | 5 | 12% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 14 | 33% |
| Environmental Science | 8 | 19% |
| Chemistry | 7 | 16% |
| Biochemistry, Genetics and Molecular Biology | 5 | 12% |
| Immunology and Microbiology | 2 | 5% |
| Other | 2 | 5% |
| Unknown | 5 | 12% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 February 2014.
All research outputs
#8,534,976
of 25,373,627 outputs
Outputs from Archives of Microbiology
#641
of 3,119 outputs
Outputs of similar age
#17,062
of 80,654 outputs
Outputs of similar age from Archives of Microbiology
#4
of 11 outputs
Altmetric has tracked 25,373,627 research outputs across all sources so far. This one is in the 43rd percentile – i.e., 43% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,119 research outputs from this source. They receive a mean Attention Score of 3.7. This one is in the 45th percentile – i.e., 45% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 80,654 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 18th percentile – i.e., 18% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 11 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.