| Title |
Let-7 microRNA inhibits the proliferation of human glioblastoma cells
|
|---|---|
| Published in |
Journal of Neuro-Oncology, July 2010
|
| DOI | 10.1007/s11060-010-0286-6 |
| Pubmed ID | |
| Authors |
Soon-Tae Lee, Kon Chu, Hyun-Jung Oh, Woo-Seok Im, Ji-Yeon Lim, Seung-Ki Kim, Cheol-Ki Park, Keun-Hwa Jung, Sang Kun Lee, Manho Kim, Jae-Kyu Roh |
| Abstract |
MicroRNAs (miRNAs) are small noncoding RNAs comprising 21-23 nucleotides that regulate gene expression by transcriptionally repressing their complementary mRNAs. In particular, let-7 miRNA has been postulated to function as a tumor suppressor in various cancer cells, but not yet in glioblastoma. In this study, we investigated the anti-tumorigenic effect of let-7 miRNA in glioblastoma cells. Human glioblastoma cells (U251 or U87 cells) were transfected with let-7 miRNA and assayed for in-vitro proliferation, migration, and in-vivo tumor formation. Transfection of let-7 miRNA reduced expression of pan-RAS, N-RAS, and K-RAS in the glioblastoma cells. Let-7 miRNA also reduced the in-vitro proliferation and migration of the cells, and reduced the sizes of the tumors produced after transplantation into nude mice. However, let-7 miRNA exerted no effect on the proliferation of normal human astrocytes. These results indicate that let-7 miRNA has an anti-tumorigenic effect on glioblastoma cells, and suggest possible use of let-7 miRNA for treating glioblastoma. |
Mendeley readers
The data shown below were compiled from readership statistics for 45 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 2% |
| Switzerland | 1 | 2% |
| Brazil | 1 | 2% |
| Unknown | 42 | 93% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 9 | 20% |
| Researcher | 9 | 20% |
| Other | 5 | 11% |
| Professor > Associate Professor | 5 | 11% |
| Student > Bachelor | 4 | 9% |
| Other | 9 | 20% |
| Unknown | 4 | 9% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 14 | 31% |
| Agricultural and Biological Sciences | 12 | 27% |
| Biochemistry, Genetics and Molecular Biology | 7 | 16% |
| Neuroscience | 2 | 4% |
| Computer Science | 1 | 2% |
| Other | 5 | 11% |
| Unknown | 4 | 9% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 November 2010.
All research outputs
#7,454,066
of 22,788,370 outputs
Outputs from Journal of Neuro-Oncology
#1,041
of 2,967 outputs
Outputs of similar age
#33,699
of 94,430 outputs
Outputs of similar age from Journal of Neuro-Oncology
#11
of 19 outputs
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So far Altmetric has tracked 2,967 research outputs from this source. They receive a mean Attention Score of 4.2. This one is in the 48th percentile – i.e., 48% of its peers scored the same or lower than it.
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