| Title |
Human Umbilical Vein Endothelial Cells Express Complement Receptor 1 (CD35) and Complement Receptor 4 (CD11c/CD18) in vitro
|
|---|---|
| Published in |
Inflammation, June 2002
|
| DOI | 10.1023/a:1015585530204 |
| Pubmed ID | |
| Authors |
Harald Langeggen, Knut Erik Berge, Egil Johnson, Geir Hetland |
| Abstract |
We have examined complement receptors on human umbilical vein endothelial cells (HUVEC) and found that they express complement receptor 1 (CR1, CD35) and complement receptor 4 (CR4, CD11c/CD18), but not complement receptor 3 (CR3, CD11b/CD18). Binding of monoclonal antibodies against CR1 (CD35) and CR4 (CD11c/CD18) to HUVEC was demonstrated by flow cytometry. The presence of the corresponding mRNAs was confirmed by reverse transcriptase-polymerase chain reaction (RT-PCR) and sequencing of the amplified cDNA fragments. When HUVEC were treated with inflammatory mediators, chemotactic agents or the secretagogue phorbol-12-myristate-13-acetate (PMA), no change in reactivity to CR1 (CD35) or CR4 (CD11c/CD18) monoclonal antibodies was detected on the surface of the cells compared with untreated cells. The presence of CR1 (CD35) and CR4 (CD1c/CD18) on HUVEC indicates that endothelial cells (EC) have the potential to bind C3b and iC3b, respectively, which both mediate biological effects in the course of complement activation. |
Mendeley readers
The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Hungary | 1 | 13% |
| Unknown | 7 | 88% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 2 | 25% |
| Student > Master | 2 | 25% |
| Student > Bachelor | 1 | 13% |
| Unknown | 3 | 38% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 2 | 25% |
| Biochemistry, Genetics and Molecular Biology | 1 | 13% |
| Immunology and Microbiology | 1 | 13% |
| Neuroscience | 1 | 13% |
| Unknown | 3 | 38% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 October 2020.
All research outputs
#8,534,528
of 25,373,627 outputs
Outputs from Inflammation
#210
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Outputs of similar age
#42,700
of 126,576 outputs
Outputs of similar age from Inflammation
#1
of 2 outputs
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