Title |
Identification of rabaptin‐5, rabex‐5, and GM130 as putative effectors of rab33b, a regulator of retrograde traffic between the Golgi apparatus and ER
|
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Published in |
Febs Letters, October 2001
|
DOI | 10.1016/s0014-5793(01)02993-3 |
Pubmed ID | |
Authors |
Rebekka Valsdottir, Hitoshi Hashimoto, Keith Ashman, Toshiaki Koda, Brian Storrie, Tommy Nilsson |
Abstract |
The role of rab33b, a Golgi-specific rab protein, was investigated. Microinjection of rab33b mutants stabilised in the GTP-specific state resulted in a marked inhibition of anterograde transport within the Golgi and in the recycling of glycosyltransferases from the Golgi to the ER, respectively. A GST-rab33b fusion protein stabilised in its GTP form was found to interact by Western blotting or mass spectroscopy with Golgi protein GM130 and rabaptin-5 and rabex-5, two rab effector molecules thought to function exclusively in the endocytic pathway. A similar binding was seen to rab1 but not to rab6, both Golgi rabs. In contrast, rab5 was as expected, shown to bind rabaptin-5 and rabex-5 as well as the endosomal effector protein EEA1 but not GM130. No binding of EEA1 was seen to any of the Golgi rabs. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Hungary | 1 | 2% |
France | 1 | 2% |
Canada | 1 | 2% |
Spain | 1 | 2% |
United States | 1 | 2% |
Unknown | 43 | 90% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 14 | 29% |
Researcher | 11 | 23% |
Student > Doctoral Student | 6 | 13% |
Professor | 4 | 8% |
Student > Bachelor | 3 | 6% |
Other | 6 | 13% |
Unknown | 4 | 8% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 22 | 46% |
Biochemistry, Genetics and Molecular Biology | 12 | 25% |
Arts and Humanities | 2 | 4% |
Chemistry | 2 | 4% |
Medicine and Dentistry | 2 | 4% |
Other | 3 | 6% |
Unknown | 5 | 10% |