| Title |
Phosphoproteomic analysis of the human pituitary
|
|---|---|
| Published in |
Pituitary, June 2006
|
| DOI | 10.1007/s11102-006-8916-x |
| Pubmed ID | |
| Authors |
Sarka Beranova-Giorgianni, Yingxin Zhao, Dominic M. Desiderio, Francesco Giorgianni |
| Abstract |
The pituitary is the central endocrine gland that regulates the functions of various target organs in the human body. Because of the pivotal regulatory role of the pituitary, it is essential to define on a global scale the components of the pituitary protein machinery, including a comprehensive characterization of the post-translational modifications of the pituitary proteins. Of particular interest is the examination of the phosphorylation status of the pituitary in health and disease. Towards the goal of global profiling of pituitary protein phosphorylation, we report here the application of the in-gel IEF-LC-MS/MS approach to the study of the pituitary phosphoproteome. The analytical strategy combined isoelectric focusing in immobilized pH gradient strips with immobilized metal ion affinity chromatography and mass spectrometry. With this method, a total of 50 phosphorylation sites were characterized in 26 proteins. Because the investigation involved primary tissue, the findings provide a direct glimpse into the phosphoprotein machinery operating within the human pituitary tissue microenvironment. |
Mendeley readers
The data shown below were compiled from readership statistics for 26 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Denmark | 1 | 4% |
| Unknown | 25 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 7 | 27% |
| Researcher | 6 | 23% |
| Student > Master | 3 | 12% |
| Student > Doctoral Student | 2 | 8% |
| Professor | 1 | 4% |
| Other | 2 | 8% |
| Unknown | 5 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 9 | 35% |
| Biochemistry, Genetics and Molecular Biology | 7 | 27% |
| Unspecified | 1 | 4% |
| Physics and Astronomy | 1 | 4% |
| Medicine and Dentistry | 1 | 4% |
| Other | 1 | 4% |
| Unknown | 6 | 23% |
Attention Score in Context
This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 March 2020.
All research outputs
#3,272,274
of 22,788,370 outputs
Outputs from Pituitary
#41
of 491 outputs
Outputs of similar age
#7,016
of 64,264 outputs
Outputs of similar age from Pituitary
#1
of 5 outputs
Altmetric has tracked 22,788,370 research outputs across all sources so far. Compared to these this one has done well and is in the 84th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 491 research outputs from this source. They receive a mean Attention Score of 3.4. This one has done particularly well, scoring higher than 91% of its peers.
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We're also able to compare this research output to 5 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them