| Title |
Anti-Giardia activity of phenolic-rich essential oils: effects of Thymbra capitata, Origanum virens, Thymus zygis subsp. sylvestris, and Lippia graveolens on trophozoites growth, viability, adherence, and ultrastructure
|
|---|---|
| Published in |
Parasitology Research, March 2010
|
| DOI | 10.1007/s00436-010-1800-7 |
| Pubmed ID | |
| Authors |
Marisa Machado, Augusto M. Dinis, Ligia Salgueiro, Carlos Cavaleiro, José B. A. Custódio, Maria do Céu Sousa |
| Abstract |
The present work evaluates the anti-Giardia activity of phenolic-rich essential oils obtained from Thymbra capitata, Origanum virens, Thymus zygis subsp. sylvestris chemotype thymol, and Lippia graveolens aromatic plants. The effects were evaluated on parasite growth, cell viability adherence, and morphology. The tested essential oils inhibited the growth of Giardia lamblia. T. capitata essential oil is the most active followed by O. virens, T. zygis subsp. sylvestris, and L. graveolens oils. The tested essential oils at IC50 (71-257) microg/ml inhibited parasite adherence (p < 0.001) since the first hour of incubation and were able to kill almost 50% of the parasites population in a time-dependent manner. The main ultrastructural alterations promoted by essential oils were deformations in typical trophozoite appearance, often roundly shape, irregular dorsal and ventral surface, presence of membrane blebs, electrodense precipitates in cytoplasm and nuclei, and internalization of flagella and ventral disc. Our data suggest that essential oils induced cell death probably by processes associated to the loss of osmoregulation caused by plasmatic membrane alterations. Experiments revealed that the essential oils did not present cytotoxic effects in mammalian cells. In conclusion, T. capitata, O. virens, T. zygis subsp. sylvestris chemotype thymol, and L. graveolens essential oils have antigiardial activity in vitro and seem to have potential for the treatment of the parasitic disease caused by the protozoan G. lamblia. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 84 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Mexico | 1 | 1% |
| Unknown | 83 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 15 | 18% |
| Researcher | 12 | 14% |
| Student > Bachelor | 11 | 13% |
| Student > Doctoral Student | 9 | 11% |
| Student > Ph. D. Student | 6 | 7% |
| Other | 10 | 12% |
| Unknown | 21 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 22 | 26% |
| Medicine and Dentistry | 8 | 10% |
| Biochemistry, Genetics and Molecular Biology | 7 | 8% |
| Chemistry | 5 | 6% |
| Veterinary Science and Veterinary Medicine | 4 | 5% |
| Other | 12 | 14% |
| Unknown | 26 | 31% |
Attention Score in Context
This research output has an Altmetric Attention Score of 16. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 February 2015.
All research outputs
#1,943,467
of 22,788,370 outputs
Outputs from Parasitology Research
#63
of 3,782 outputs
Outputs of similar age
#7,194
of 93,472 outputs
Outputs of similar age from Parasitology Research
#2
of 21 outputs
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So far Altmetric has tracked 3,782 research outputs from this source. They receive a mean Attention Score of 2.7. This one has done particularly well, scoring higher than 98% of its peers.
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