| Title |
Protein profiling as early detection biomarkers for TiO2 nanoparticle toxicity in Daphnia magna
|
|---|---|
| Published in |
Ecotoxicology, March 2018
|
| DOI | 10.1007/s10646-018-1907-7 |
| Pubmed ID | |
| Authors |
Paula Sá-Pereira, Mário S. Diniz, Liliana Moita, Teresa Pinheiro, Elsa Mendonça, Susana M. Paixão, Ana Picado |
| Abstract |
The mode of action for nanoparticle (NP) toxicity in aquatic organisms is not yet fully understood. In this work, a strategy other than toxicity testing was applied to Daphnia magna exposed to TiO2-NPs: the use of nuclear microscopy and the assessment of protein profile. D. magna is a keystone species broadly used as a model system in ecotoxicology. Titanium (Ti) was found in the D. magna digestive tract, mainly in the gut. The penetration of Ti into the epithelial region was greater at higher exposure levels and also observed in eggs in the brood pouch. The protein profile of individuals exposed to different concentrations showed that 2.8 and 5.6 mg/L TiO2-NP concentrations induced an over-expression of the majority of proteins, in particular proteins with molecular weight of ∼120, 85 and 15 kDa, while 11.2 mg/L TiO2-NP had an inhibitory effect on protein expression. The Matrix-assisted laser desorption ionization with tandem time of flight mass spectrometry (MALDI-TOF/TOF MS) analysis of these proteins consistently identified them as vitellogenin (Vtg)-like proteins, associated with enzymes involved in redox balance. These results indicate that Vtg-like proteins are up-regulated in D. magna exposed to TiO2-NPs. Vitellogenesis is associated with the reproduction system, suggesting that TiO2-NP exposure can impair reproduction by affecting this process. The precise mode of action of TiO2-NPs is still unclear and the results from this study are a first attempt to identify specific proteins as potential markers of TiO2-NP toxicity in D. magna, providing useful information for future research. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 33 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 33 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 7 | 21% |
| Student > Master | 6 | 18% |
| Professor > Associate Professor | 3 | 9% |
| Student > Doctoral Student | 2 | 6% |
| Student > Ph. D. Student | 2 | 6% |
| Other | 4 | 12% |
| Unknown | 9 | 27% |
| Readers by discipline | Count | As % |
|---|---|---|
| Environmental Science | 7 | 21% |
| Chemistry | 5 | 15% |
| Agricultural and Biological Sciences | 4 | 12% |
| Biochemistry, Genetics and Molecular Biology | 2 | 6% |
| Medicine and Dentistry | 1 | 3% |
| Other | 3 | 9% |
| Unknown | 11 | 33% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 September 2018.
All research outputs
#15,495,840
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Outputs from Ecotoxicology
#658
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Outputs of similar age
#211,608
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Outputs of similar age from Ecotoxicology
#21
of 37 outputs
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