| Title |
Reconstituted Cl− pump protein: A novel ion(Cl−)-motive ATPase
|
|---|---|
| Published in |
Journal of Bioenergetics and Biomembranes, December 1996
|
| DOI | 10.1007/bf02110436 |
| Pubmed ID | |
| Authors |
George A. Gerencser, Karnam R. Purushotham |
| Abstract |
Cl- absorption by the Aplysia californica foregut is effected through an active Cl- transport mechanism located in the basolateral membrane of the epithelial absorptive cells. These basolateral membranes contain both Cl(-)-stimulated ATPase and ATP-dependent Cl- transport activities which can be incorporated into liposomes via reconstitution. Utilizing the proteoliposomal preparation, it was demonstrated that ATP, and its subsequent hydrolysis, Mg2+, Cl-, and a pH optimum of 7.8 were required to generate maximal intraliposomal Cl- accumulation, electrical negativity, and ATPase activity. Additionally, an inwardly-directed valinomycin-induced K+ diffusion potential, making the liposome interior electrically positive, enhanced both ATP-driven Cl- accumulation and electrical potential while an outwardly-directed valinomycin-induced K+ diffusion potential, making the liposome interior electrically negative, decreased both ATP-driven Cl- accumulation and electrical potential compared with proteoliposomes lacking the ionophore. Either orthovanadate or p-chloromercurobenzene sulfonate inhibited both the ATP-dependent intraliposomal Cl- accumulation, intraliposomal negative potential difference, and also Cl(-)-stimulated ATPase activity. Both aspects of Cl- pump transport kinetics and its associated catalytic component kinetics were the first obtained utilizing a reconstituted transporter protein. These results strongly support the hypothesis that Cl(-)-ATPase actively transports Cl- by an electrogenic process. |
Mendeley readers
The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 3 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 2 | 67% |
| Student > Master | 1 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 2 | 67% |
| Biochemistry, Genetics and Molecular Biology | 1 | 33% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 May 2013.
All research outputs
#7,926,100
of 23,867,274 outputs
Outputs from Journal of Bioenergetics and Biomembranes
#105
of 466 outputs
Outputs of similar age
#20,414
of 94,247 outputs
Outputs of similar age from Journal of Bioenergetics and Biomembranes
#1
of 9 outputs
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So far Altmetric has tracked 466 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 39th percentile – i.e., 39% of its peers scored the same or lower than it.
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