| Title |
Controlled depolymerisation assessed by analytical ultracentrifugation of low molecular weight chitosan for use in archaeological conservation
|
|---|---|
| Published in |
European Biophysics Journal, March 2018
|
| DOI | 10.1007/s00249-018-1290-6 |
| Pubmed ID | |
| Authors |
Jennifer M. K. Wakefield, Richard B. Gillis, Gary G. Adams, Caitlin M. A. McQueen, Stephen E. Harding |
| Abstract |
The heterogeneity and molecular weight of a chitosan of low molecular weight (molar mass) and low degree of acetylation (0.1) for potential use as a consolidant for decayed archaeological wood were examined by sedimentation velocity and sedimentation equilibrium in the analytical ultracentrifuge before and after depolymerisation. Sedimentation velocity before depolymerisation revealed a uniform distribution of sedimentation coefficient with little concentration dependence. SEDFIT-MSTAR analysis revealed a weight average molecular weight Mw of (14.2 ± 1.2) kDa, and polydispersity index of ~ 1.2. Further analysis using MULTISIG revealed a distribution of material between 2 and 20 kDa and consistent with the weight average Mw. Controlled depolymerisation using hydrogen peroxide and ultra-violet radiation in an acetic acid medium reduced this to (4.9 ± 0.7) kDa, with a similar polydispersity. The depolymerised material appears to be within the range that has been predicted to fully penetrate into archaeological wood. The consequences for this finding and the use of the analytical ultracentrifuge in wood conservation strategies are considered. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 27 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 27 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 7 | 26% |
| Student > Ph. D. Student | 5 | 19% |
| Student > Master | 3 | 11% |
| Student > Bachelor | 2 | 7% |
| Unspecified | 1 | 4% |
| Other | 3 | 11% |
| Unknown | 6 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Arts and Humanities | 6 | 22% |
| Chemistry | 4 | 15% |
| Engineering | 2 | 7% |
| Chemical Engineering | 1 | 4% |
| Agricultural and Biological Sciences | 1 | 4% |
| Other | 7 | 26% |
| Unknown | 6 | 22% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 January 2023.
All research outputs
#6,644,068
of 23,477,147 outputs
Outputs from European Biophysics Journal
#91
of 496 outputs
Outputs of similar age
#126,291
of 360,937 outputs
Outputs of similar age from European Biophysics Journal
#4
of 13 outputs
Altmetric has tracked 23,477,147 research outputs across all sources so far. This one has received more attention than most of these and is in the 70th percentile.
So far Altmetric has tracked 496 research outputs from this source. They receive a mean Attention Score of 3.1. This one has done well, scoring higher than 81% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 360,937 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 64% of its contemporaries.
We're also able to compare this research output to 13 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 69% of its contemporaries.