Title |
Biphenyl synthase from yeast-extract-treated cell cultures of Sorbus aucuparia
|
---|---|
Published in |
Planta, November 2003
|
DOI | 10.1007/s00425-003-1144-y |
Pubmed ID | |
Authors |
Benye Liu, Till Beuerle, Tim Klundt, Ludger Beerhues |
Abstract |
Biphenyls and dibenzofurans are the phytoalexins of the Maloideae, a subfamily of the economically important Rosaceae. The biphenyl aucuparin accumulated in Sorbus aucuparia L. cell cultures in response to yeast extract treatment. Incubation of cell-free extracts from challenged cell cultures with benzoyl-CoA and malonyl-CoA led to the formation of 3,5-dihydroxybiphenyl. This reaction was catalysed by a novel polyketide synthase, which will be named biphenyl synthase. The most efficient starter substrate for the enzyme was benzoyl-CoA. Relatively high activity was also observed with 2-hydroxybenzoyl-CoA but, instead of the corresponding biphenyl, the derailment product 2-hydroxybenzoyltriacetic acid lactone was formed. |
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Geographical breakdown
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Unknown | 21 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 4 | 19% |
Professor | 3 | 14% |
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Researcher | 1 | 5% |
Other | 0 | 0% |
Unknown | 7 | 33% |
Readers by discipline | Count | As % |
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Chemistry | 3 | 14% |
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Unknown | 7 | 33% |