| Title |
Identification and properties of UDP-glucose: Cyanidin-3-O-glucosyltransferase isolated from petals of the red campion (Silene dioica)
|
|---|---|
| Published in |
Biochemical Genetics, December 1978
|
| DOI | 10.1007/bf00484525 |
| Pubmed ID | |
| Authors |
John Kamsteeg, Jan van Brederode, Gerrit van Nigtevecht |
| Abstract |
An enzyme catalyzing the transfer of the glucosyl moiety of UDP-glucose to the 3-hydroxyl group of cyanidin has been demonstrated in petal extracts of Silene dioica mutants with cyanidin-3-O-glucoside in the petals. This transferase activity was also present in young rosette leaves and calyces of these plants. The highest glucosyltransferase activity was found in petals of opening flowers of young plants. The enzyme was purified ninetyfold by PVP and Sephadex chromatography. The glucosyltransferase had a pH optimum of 7.5, had a "true Km value" of 4.1 x 10(-4) M for UDP-glucose and 0.4 x 10(-4) M for cyanidin chloride, and was not stimulated by divalent metal ions. Both p-chloromercuribenzoate and HgCl2 inhibited the enzyme activity. Pelargonidin chloride and delphinidin chloride at reduced rates also served as substrates. The enzyme did not catalyze the glucosylation of the 3-hydroxyl group of flavonols or the 5-hydroxyl group of anthocyanins. ADP-glucose could not serve as a glucosyl donor. The results of Sephadex G150 chromatography suggest that the glucosyltransferase can exist as dimer of about 125,000 daltons and as active monomers of 60,000 daltons. The genetic control of the glucosyltransferase activity is discussed. |
Mendeley readers
The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Australia | 1 | 9% |
| Unknown | 10 | 91% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 4 | 36% |
| Professor | 2 | 18% |
| Student > Doctoral Student | 1 | 9% |
| Researcher | 1 | 9% |
| Professor > Associate Professor | 1 | 9% |
| Other | 0 | 0% |
| Unknown | 2 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 3 | 27% |
| Biochemistry, Genetics and Molecular Biology | 3 | 27% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 9% |
| Business, Management and Accounting | 1 | 9% |
| Medicine and Dentistry | 1 | 9% |
| Other | 0 | 0% |
| Unknown | 2 | 18% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 March 2013.
All research outputs
#7,454,066
of 22,788,370 outputs
Outputs from Biochemical Genetics
#61
of 471 outputs
Outputs of similar age
#4,072
of 26,102 outputs
Outputs of similar age from Biochemical Genetics
#1
of 4 outputs
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